Evidence map›Paper›PMID 32857376›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Ex Vivo Techniques to Study Heart Regeneration in Zebrafish.

Sierra Duca, Jingli Cao

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 83% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Sierra DucaDepartment of Cell and Developmental Biology, Cardiovascular Research Institute, Weill Cornell Medical College, Cornell University, New York, NY, USA.
Jingli CaoDepartment of Cell and Developmental Biology, Cardiovascular Research Institute, Weill Cornell Medical College, Cornell University, New York, NY, USA. jic4001@med.cornell.edu.
Cornell University · US

Funding

American Heart Association-American Stroke Association 18CDA34110108
6 · The paper itself

Abstract

Due to its pronounced regenerative capacity, the zebrafish heart represents an advantageous model system for exploring the cellular and molecular mechanisms of cardiac regeneration. Upon injury, the epicardium, the outermost mesothelial tissue layer of vertebrate hearts, serves dual purposes in the regenerating heart as both a signaling center and a source for crucial cell types. Traditional in vivo genetic approaches to study heart regeneration can be time consuming and are not applicable to large-scale approaches and live surveillance of cellular behaviors. Here, we demonstrate ex vivo methods to culture, maintain, and study the regenerative responses of epicardial tissue in excised zebrafish hearts. Epicardial cell proliferation and migration are monitored in real time after uninjured or injured hearts are excised, washed, and cultured for up to 30 days. In addition to these techniques, we describe ex vivo genetic ablation of the epicardium, cell proliferation assays, partial ventricular explant culturing, and chemical screening.

Indexed as

RegenerationAnimalsAnimals, Genetically ModifiedAnti-Infective AgentsCell MovementCell ProliferationHeartHigh-Throughput Screening AssaysMetronidazoleMyocytes, CardiacPericardiumZebrafishZebrafish ProteinsAnti-Infective AgentsMetronidazoleZebrafish ProteinsChemical screeningEpicardiumHeart explant cultureHeart regenerationLive imagingZebrafish

Identifiers

PMID32857376
OpenAlexW3082108943

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.