ArticleJournal of veterinary internal medicine2020
Effects of metronidazole on the fecal microbiome and metabolome in healthy dogs.
Article in Journal of veterinary internal medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 125 papers.
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Who cites it
125 citing papers in PubMed, 204 citations in OpenAlex.
- A new highly digestible prescription diet containingFrontiers in veterinary science · 2025Trial
- Fecal Microbiota Transplantation in Dogs and Cats: Evidence for Gastrointestinal and Emerging Extra-Intestinal Applications.Animals : an open access journal from MDPI · 2026Review
- Sustained Tear Film Exposure After Parenteral Cefovecin Administration in Dogs: Pharmacokinetics and Relevance to Canine Bacterial Keratitis.Veterinary ophthalmology · 2026Article
- Effect of low dose azithromycin on the fecal microbiome and fecal bile acids in healthy dogs.Journal of veterinary internal medicine · 2026Article
- Neutrophilic inflammatory enteropathy in 27 dogs: a retrospective descriptive study.Journal of veterinary internal medicine · 2026Article
- The Impact of Vaginal Bacteria and Antimicrobial Treatment on Pregnancy Outcomes in Healthy Breeding Bitches.Antibiotics (Basel, Switzerland) · 2026Article
- An Ecological Framework for Interpreting the Canine Gut Microbiome.Animals : an open access journal from MDPI · 2026Article
- Temporal variability is an inherent feature of the healthy canine microbiome assessed by full-length 16S rRNA gene sequencing.Animal microbiome · 2026Article
- Associations between early life exposure to parvovirus and antibiotic treatment and the occurrence and severity of later chronic enteropathy in dogs.The Veterinary record · 2026Article
- Dual probiotic intervention with Lactiplantibacillus plantarum Kmiate-6 and Pediococcus acidilactici Kmiate-15 improves periodontal health and oral microbiota balance in dogs.Animal microbiome · 2026Article
- A Functional Metabolomics Framework to Track Microbiome Drug Metabolism.bioRxiv : the preprint server for biology · 2026Article
- Microbiota Transplantation as a Future Novel Therapeutic Strategy Approach.Diseases (Basel, Switzerland) · 2026Review
- An exploratory study of behavioral, cognitive, physiological, and microbiota profiles in senior dogs.Frontiers in behavioral neuroscience · 2026Article
- Risk factors for recurrence of Giardia duodenalis infection in dogs: a case-control study.The Journal of small animal practice · 2026Article
- Understanding the rationale for metronidazole use in dogs and cats.The Journal of small animal practice · 2025Article
- Effects ofMicroorganisms · 2025Article
- The gut microbiota of Labrador retriever puppies: a longitudinal cohort study.Animal microbiome · 2025Article
- Impacts of Vincristine and Prednisolone Chemotherapy on the Canine Gut Microbiota in Dogs Undergoing Treatment for Lymphoma.Veterinary and comparative oncology · 2025Article
- In vitro fermentation characteristics of dietary fibers using fecal inocula from dogs treated with metronidazole.Animal microbiome · 2025Article
- Multimodal sensory overload in dopamine-deficient larval zebrafish leads to paradoxical kinesia.Research square · 2025Article
65 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMetronidazole has a substantial impact on the gut microbiome. However, the recovery of the microbiome after discontinuation of administration, and the metabolic consequences of such alterations have not been investigated to date.
objectivesTo describe the impact of 14-day metronidazole administration, alone or in combination with a hydrolyzed protein diet, on fecal microbiome, metabolome, bile acids (BAs), and lactate production, and on serum metabolome in healthy dogs. ANIMALS: Twenty-four healthy pet dogs.
methodsProspective, nonrandomized controlled study. Dogs fed various commercial diets were divided in 3 groups: control group (no intervention, G1); group receiving hydrolyzed protein diet, followed by metronidazole administration (G2); and group receiving metronidazole only (G3). Microbiome composition was evaluated with sequencing of 16S rRNA genes and quantitative polymerase chain reaction (qPCR)-based dysbiosis index. Untargeted metabolomics analysis of fecal and serum samples was performed, followed by targeted assays for fecal BAs and lactate.
resultsNo changes were observed in G1, or G2 during diet change. Metronidazole significantly changed microbiome composition in G2 and G3, including decreases in richness (P < .001) and in key bacteria such as Fusobacteria (q < 0.001) that did not fully resolve 4 weeks after metronidazole discontinuation. Fecal dysbiosis index was significantly increased (P < .001). Those changes were accompanied by increased fecal total lactate (P < .001), and decreased secondary BAs deoxycholic acid and lithocholic acid (P < .001). CONCLUSION AND CLINICAL IMPORTANCE: Our results indicate a minimum 4-week effect of metronidazole on fecal microbiome and metabolome, supporting a cautious approach to prescription of metronidazole in dogs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.