Evidence map›Paper›PMID 32856205›Full record

ArticleJournal of molecular neuroscience : MN2021

ClC-2-like Chloride Current Alterations in a Cell Model of Spinal and Bulbar Muscular Atrophy, a Polyglutamine Disease.

Vladimir A Martínez-Rojas, Aura M Jiménez-Garduño, Daniela Michelatti, Laura Tosatto, Marta Marchioretto, Daniele Arosio, Manuela Basso, Maria Pennuto, Carlo Musio

Abstract read
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In one paragraph

Article in Journal of molecular neuroscience : MN, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.6field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 19 citations in OpenAlex.

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  8. Protective Effects of PACAP in a Rat Model of Diabetic Neuropathy.International journal of molecular sciences · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Vladimir A Martínez-Rojas *Institute of Biophysics (IBF), Trento Unit, National Research Council (CNR) & LabSSAH, Bruno Kessler Foundation (FBK), Trento, Italy.
Aura M Jiménez-Garduño *Institute of Biophysics (IBF), Trento Unit, National Research Council (CNR) & LabSSAH, Bruno Kessler Foundation (FBK), Trento, Italy.
Daniela Michelatti *Institute of Biophysics (IBF), Trento Unit, National Research Council (CNR) & LabSSAH, Bruno Kessler Foundation (FBK), Trento, Italy.
Laura TosattoInstitute of Biophysics (IBF), Trento Unit, National Research Council (CNR) & LabSSAH, Bruno Kessler Foundation (FBK), Trento, Italy.
Marta MarchiorettoInstitute of Biophysics (IBF), Trento Unit, National Research Council (CNR) & LabSSAH, Bruno Kessler Foundation (FBK), Trento, Italy.
Daniele ArosioInstitute of Biophysics (IBF), Trento Unit, National Research Council (CNR) & LabSSAH, Bruno Kessler Foundation (FBK), Trento, Italy.
Manuela BassoCIBIO Department, Laboratory of Transcriptional Neurobiology, University of Trento, Trento, Italy.
Maria PennutoDepartment of Biomedical Sciences, University of Padova, Padova, Italy.
Carlo MusioInstitute of Biophysics (IBF), Trento Unit, National Research Council (CNR) & LabSSAH, Bruno Kessler Foundation (FBK), Trento, Italy. carlo.musio@cnr.it.ORCID http://orcid.org/0000-0003-2106-9931
Fondazione Bruno Kessler · ITUniversity of Trento · ITUniversity of Padua · IT

Funding

Association Française contre les Myopathies 22221Fondazione Cassa Di Risparmio Di Trento E Rovereto Bando Post-docFondazione Cassa Di Risparmio Di Trento E Rovereto Bando Post-DocFondazione Telethon TCP12013Muscular Dystrophy Association 479363Provincia Autonoma di Trento (IT) Great project "MaDEleNA"Università degli Studi di Trento Bando Progetti Strategici di Ateneo
6 · The paper itself

Abstract

Spinal and bulbar muscular atrophy (SBMA) is a neuromuscular disease caused by expansions of a polyglutamine (polyQ) tract in the androgen receptor (AR) gene. SBMA is associated with the progressive loss of lower motor neurons, together with muscle weakness and atrophy. PolyQ-AR is converted to a toxic species upon binding to its natural ligands, testosterone, and dihydrotestosterone (DHT). Our previous patch-clamp studies on a motor neuron-derived cell model of SBMA showed alterations in voltage-gated ion currents. Here, we identified and characterized chloride currents most likely belonging to the chloride channel-2 (ClC-2) subfamily, which showed significantly increased amplitudes in the SBMA cells. The treatment with the pituitary adenylyl cyclase-activating polypeptide (PACAP), a neuropeptide with a proven protective effect in a mouse model of SBMA, recovered chloride channel current alterations in SBMA cells. These observations suggest that the CIC-2 currents are affected in SBMA, an alteration that may contribute and potentially determine the pathophysiology of the disease.

Indexed as

Action PotentialsAnimalsBulbo-Spinal Atrophy, X-LinkedCells, CulturedChloride ChannelsCLC-2 Chloride ChannelsMiceNeuronsPituitary Adenylate Cyclase-Activating PolypeptideChloride ChannelsCLC-2 Chloride ChannelsClcn2 protein, mousePituitary Adenylate Cyclase-Activating PolypeptideChloride currentsClC-2 channelsPACAPPatch-clampPolyQ-AR cellsSpinal and bulbar muscular atrophy (SBMA)

Identifiers

PMID32856205
OpenAlexW3081987483

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.