Evidence map›Paper›PMID 32854428›Full record

ArticleInternational journal of molecular sciences2020

Expansion of Human Limbal Epithelial Stem/Progenitor Cells Using Different Human Sera: A Multivariate Statistical Analysis.

Raquel Hernáez-Moya, Sheyla González, Arantza Urkaregi, Jose Ignacio Pijoan, Sophie X Deng, Noelia Andollo

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
  4. Review
  5. Multiple Stem/Progenitor Cells Isolated from the Limbus.Current stem cell research & therapy · 2025
    Review
  6. Article
  7. Concise Review: Bioengineering of Limbal Stem Cell Niche.Bioengineering (Basel, Switzerland) · 2023
    Review
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Raquel Hernáez-MoyaDepartment of Cell Biology and Histology, School of Medicine and Nursing, Biocruces Bizkaia Health Research Institute, University of the Basque Country UPV/EHU, 48940 Leioa, Bizkaia, Spain.ORCID 0000-0002-8684-8306
Sheyla GonzálezCornea Division, Stein Eye Institute, University of California, Los Angeles, CA 90095, USA.
Arantza UrkaregiDepartment of Applied Mathematics and Statistics and Operational Research, Biocruces Bizkaia Health Research Institute, University of the Basque Country UPV/EHU, 48940 Leioa, Bizkaia, Spain.
Jose Ignacio PijoanClinical Epidemiology Unit, Cruces University Hospital, Biocruces Bizkaia Health Research Institute, 48903 Barakaldo, Bizkaia, Spain.ORCID 0000-0002-5974-5280
Sophie X DengCornea Division, Stein Eye Institute, University of California, Los Angeles, CA 90095, USA.
Noelia AndolloDepartment of Cell Biology and Histology, School of Medicine and Nursing, Biocruces Bizkaia Health Research Institute, University of the Basque Country UPV/EHU, 48940 Leioa, Bizkaia, Spain.ORCID 0000-0002-8918-6395

Funding

Ex Vivo Expansion of Human Limbal Stem Cells for TransplantationR01EY021797 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Sophie Deng · 2012 to 2026
$5.1M
Basque Foundation for Health Research and Innovation BIOEF BIO14/TP/002California Institute for Regenerative Medicine TR- TR2-01768 and CLIN1-08686Jesus de Gangoiti Barrera foundation Fellowships for R. H-M.Mutua Madrileña Foundation FMM11/02NEI NIH HHS R01 EY021797NEI NIH HHS R01EY021797Research to Prevent Blindness to the Department of Ophthalmology, University of California, Los Angeles unrestricted grantUniversity of the Basque Country UPV/EHU Fellowships for R. H-M.
6 · The paper itself

Abstract

Transplantation of human cultured limbal epithelial stem/progenitor cells (LESCs) has demonstrated to restore the integrity and functionality of the corneal surface in about 76% of patients with limbal stem cell deficiency. However, there are different protocols for the expansion of LESCs, and many of them use xenogeneic products, being a risk for the patients' health. We compared the culture of limbal explants on the denuded amniotic membrane in the culture medium-supplemental hormone epithelial medium (SHEM)-supplemented with FBS or two differently produced human sera. Cell morphology, cell size, cell growth rate, and the expression level of differentiation and putative stem cell markers were examined. Several bioactive molecules were quantified in the human sera. In a novel approach, we performed a multivariate statistical analysis of data to investigate the culture factors, such as differently expressed molecules of human sera that specifically influence the cell phenotype. Our results showed that limbal cells cultured with human sera grew faster and contained similar amounts of small-sized cells, higher expression of the protein p63α, and lower of cytokeratin K12 than FBS cultures, thus, maintaining the stem/progenitor phenotype of LESCs. Furthermore, the multivariate analysis provided much data to better understand the obtaining of different cell phenotypes as a consequence of the use of different culture methodologies or different culture components.

Indexed as

AdultBiomarkersCell Culture TechniquesCell DifferentiationCell ProliferationCells, CulturedCell SizeCulture MediaEpithelium, CornealHumansKeratin-12Limbus CorneaeMiddle AgedMultivariate AnalysisSerumStem CellsBiomarkersCulture MediaKeratin-12TP63 protein, humanTranscription FactorsTumor Suppressor Proteinscell therapyexplants culturehuman amniotic membrane (HAM)human serum (HS)limbal epithelial stem/progenitor cells (LESC)limbal stem cell deficiency (LSCD)multivariate statistical analysispartial least squares discriminant analysis (PLS-DA)principal component analysis (PCA) combined with clusters analysisserum derived from plasma rich in growth factors (s-PRGF)xenogeneic-free cell expansion

Identifiers

PMID32854428
PMCPMC7503296

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.