ArticleInternational journal of environmental research and public health2020
Validation and Application of a Derivatization-Free RP-HPLC-DAD Method for the Determination of Low Molecular Weight Salivary Metabolites.
Article in International journal of environmental research and public health, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Sex-Specific Salivary Biochemical Composition in a Healthy Population.International journal of molecular sciences · 2026Article
- A Comprehensive Review of Advanced Lactate Biosensor Materials, Methods, and Applications in Modern Healthcare.Sensors (Basel, Switzerland) · 2025Review
- A Moderate Intake of Beer Improves Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) in a High-Fat Diet (HFD)-Induced Mouse Model.Molecules (Basel, Switzerland) · 2024Article
- Development and validation of a non-invasive method for quantifying amino acids in human saliva.RSC advances · 2024Article
- The Role of the Preanalytical Step for Human Saliva Analysis via Vibrational Spectroscopy.Metabolites · 2023Article
- Fast, Direct Dihydrouracil Quantitation in Human Saliva: Method Development, Validation, and Application.International journal of environmental research and public health · 2022Article
- Unraveling the Extracellular Metabolism of Immortalized Hippocampal Neurons Under Normal Growth Conditions.Frontiers in chemistry · 2021Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Saliva is an interesting, non-conventional, valuable diagnostic fluid. It can be collected using standardized sampling device; thus, its sampling is easy and non-invasive, it contains a variety of organic metabolites that reflect blood composition. The aim of this study was to validate a user-friendly method for the simultaneous determination of low molecular weight metabolites in saliva. We have optimized and validated a high throughput, direct, low-cost reversed phase liquid chromatographic method with diode array detection method without any pre- or post-column derivatization. We indexed salivary biomolecules in 35 whole non-stimulated saliva samples collected in 8 individuals in different days, including organic acids and amino acids and other carbonyl compounds. Among these, 16 whole saliva samples were collected by a single individual over three weeks before, during and after treatment with antibiotic in order to investigate the dynamics of metabolites. The concentrations of the metabolites were compared with the literature data. The multianalyte method here proposed requires a minimal sample handling and it is cost-effectiveness as it makes possible to analyze a high number of samples with basic instrumentation. The identification and quantitation of salivary metabolites may allow the definition of potential biomarkers for non-invasive "personal monitoring" during drug treatments, work out, or life habits over time.
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