ArticleMetabolites2020
A Metabolomics Workflow for Analyzing Complex Biological Samples Using a Combined Method of Untargeted and Target-List Based Approaches.
Article in Metabolites, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed.
- Chemical Profiling and In Vitro Cytotoxicity Assessment of Commercial Red Yeast Rice Ingredients.Foods (Basel, Switzerland) · 2026Article
- Untargeted Metabolomic Analysis of Cell-Free Supernatants (CFSs) from Different Clinical Isolates ofJournal of fungi (Basel, Switzerland) · 2026Article
- Lipid and Metabolite Annotation Using Lipid Data Analyzer.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Metabolomic insights of cultured meat compared to conventional meat.Scientific reports · 2025Article
- Metabolomic and Lipidomic Alterations in Patients with Atopic Dermatitis with Dupilumab-Associated Ocular Surface Disease.JID innovations : skin science from molecules to population health · 2025Article
- Critical assessment of quenching and extraction/sample preparation methods for microorganisms in metabolomics.Metabolomics : Official journal of the Metabolomic Society · 2025Review
- Multi-omics profiling in spinal muscular atrophy (SMA): investigating lipid and metabolic alterations through longitudinal CSF analysis of Nusinersen-treated patients.Journal of neurology · 2025Article
- Identification of Bioactive Substances Derived from the Probiotic-Induced Bioconversion ofMicroorganisms · 2024Article
- An Untargeted Metabolomic Analysis ofMicroorganisms · 2024Article
- Untargeted Plasma Metabolomic Profiling in Patients with Depressive Disorders: A Preliminary Study.Metabolites · 2024Article
- Protective signature of xanthohumol on cognitive function of APP/PS1 mice: a urine metabolomics approach by age.Frontiers in pharmacology · 2024Article
- Fluid shear stress induces a shift from glycolytic to amino acid pathway in human trophoblasts.Cell & bioscience · 2023Article
- Ultraviolet exposure regulates skin metabolome based on the microbiome.Scientific reports · 2023Article
- Untargeted Metabolomics to Characterize the Urinary Chemical Landscape of E-Cigarette Users.Chemical research in toxicology · 2023Article
- Biosynthetic regulatory network of flavonoid metabolites in stems and leaves of Salvia miltiorrhiza.Scientific reports · 2022Article
- Changes in the Cerebrospinal Fluid and Plasma Lipidome in Patients with Rett Syndrome.Metabolites · 2022Article
- Applications of spatially resolved omics in the field of endocrine tumors.Frontiers in endocrinology · 2022Review
- Untargeted metabolomics of pulmonary tuberculosis patient serum reveals potential prognostic markers of both latent infection and outcome.Frontiers in public health · 2022Article
- Review
- Recurrent Topics in Mass Spectrometry-Based Metabolomics and Lipidomics-Standardization, Coverage, and Throughput.Analytical chemistry · 2021Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
In the highly dynamic field of metabolomics, we have developed a method for the analysis of hydrophilic metabolites in various biological samples. Therefore, we used hydrophilic interaction chromatography (HILIC) for separation, combined with a high-resolution mass spectrometer (MS) with the aim of separating and analyzing a wide range of compounds. We used 41 reference standards with different chemical properties to develop an optimal chromatographic separation. MS analysis was performed with a set of pooled biological samples human cerebrospinal fluid (CSF), and human plasma. The raw data was processed in a first step with Compound Discoverer 3.1 (CD), a software tool for untargeted metabolomics with the aim to create a list of unknown compounds. In a second step, we combined the results obtained with our internally analyzed reference standard list to process the data along with the Lipid Data Analyzer 2.6 (LDA), a software tool for a targeted approach. In order to demonstrate the advantages of this combined target-list based and untargeted approach, we not only compared the relative standard deviation (%RSD) of the technical replicas of pooled plasma samples (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.