ArticlePloS one2020
Evaluation of transplantation sites for human intestinal organoids.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Large-scale and innervated functional human gut tissues for transplantation via transient spheroid confinement.Nature biomedical engineering · 2026Article
- Building and regenerating intestines by manipulating intestinal stem cells.Nature reviews. Gastroenterology & hepatology · 2026Review
- Mapping mesenchymal diversity in the human small intestine and organoids.Nature cell biology · 2026Article
- Advances and applications of gut organoids: modeling intestinal diseases and therapeutic development.Life medicine · 2025Review
- Innervation of Human Intestinal Organoids.Journal of visualized experiments : JoVE · 2025Article
- Controlled aggregative assembly to form self-organizing macroscopic human intestine from induced pluripotent stem cells.Cell reports methods · 2024Article
- Human pluripotent stem cell-derived organoids repair damaged bowel in vivo.Cell stem cell · 2024Article
- Cutting-edge regenerative therapy for Hirschsprung disease and its allied disorders.Surgery today · 2024Review
- Development of functional resident macrophages in human pluripotent stem cell-derived colonic organoids and human fetal colon.Cell stem cell · 2023Article
- Therapeutic Potential of Human Intestinal Organoids in Tissue Repair Approaches in Inflammatory Bowel Diseases.Inflammatory bowel diseases · 2023Review
- Transplanted human intestinal organoids: a resource for modeling human intestinal development.Development (Cambridge, England) · 2023Article
- Evaluation of Murine Host Sex as a Biological Variable in Transplanted Human Intestinal Organoid Development.Digestive diseases and sciences · 2022Article
- Suspension culture in a rotating bioreactor for efficient generation of human intestinal organoids.Cell reports methods · 2022Article
- Investigation of murine host sex as a biological variable in epithelial barrier function and muscle contractility in human intestinal organoids.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Aggregation of cryopreserved mid-hindgut endoderm for more reliable and reproducible hPSC-derived small intestinal organoid generation.Stem cell reports · 2022Article
- Eicosatetraynoic Acid and Butyrate Regulate Human Intestinal Organoid Mitochondrial and Extracellular Matrix Pathways Implicated in Crohn's Disease Strictures.Inflammatory bowel diseases · 2022Article
- In Vivo Transplantation of Human Intestinal Organoids Enhances Select Tight Junction Gene Expression.The Journal of surgical research · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Our group has developed two transplantation models for the engraftment of Human Intestinal Organoids (HIOs): the renal subcapsular space (RSS) and the mesentery each with specific benefits for study. While engraftment at both sites generates laminated intestinal structures, a direct comparison between models has not yet been performed. Embryonic stem cells were differentiated into HIOs, as previously described. HIOs from the same batch were transplanted on the same day into either the RSS or mesentery. 10 weeks were allowed for engraftment and differentiation, at which time they were harvested and assessed. Metrics for comparison included: mortality, engraftment rate, gross size, number and grade of lumens, and expression of markers specific to epithelial differentiation, mesenchymal differentiation, and carbohydrate metabolism. Mortality was significantly increased when undergoing mesentery transplantation, however engraftment was significantly higher. Graft sizes were similar between groups. Morphometric parameters were similar between groups, however m-tHIOs presented with significantly fewer lumens than k-tHIO. Transcript and protein level expression of markers specific to epithelial differentiation, mesenchymal differentiation, and carbohydrate metabolism were similar between groups. Transplantation into both sites yields viable tissue of similar quality based on our assessments with enhanced engraftment and a dominant lumen for uniform study benefiting the mesenteric site and survival benefiting RSS.
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Registered trials
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