Evidence map›Paper›PMID 32852762›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Production and Analysis of Human Primordial Germ Cell-Like Cells.

Shino Mitsunaga, Keiko Shioda, Jacob H Hanna, Kurt J Isselbacher, Toshi Shioda

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Shino MitsunagaCenter for Cancer Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA.
Keiko ShiodaCenter for Cancer Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA.
Jacob H HannaDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
Kurt J IsselbacherCenter for Cancer Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA.
Toshi ShiodaCenter for Cancer Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA. shioda@helix.mgh.harvard.edu.
Harvard University · USMassachusetts General Hospital · USWeizmann Institute of Science · IL

Funding

Transgenerational obesity caused by ancestral exposure to obesogens in utero: changes in germline genomic architecture, roles of gonadal somatic cells, and metabolomic analysis of sexual dimorphismR01ES023316 · NIEHS · UNIVERSITY OF CALIFORNIA-IRVINE · PI BRUCE BLUMBERG, TOSHIHIRO SHIODA · 2013 to 2026
$8.3M
Interactions between prenatal obesogen exposure and Total Western diet lead to a transgenerational thrifty phenotype: functional and epigenomic analysis of effects in fat and liverR01ES031139 · NIEHS · UNIVERSITY OF CALIFORNIA-IRVINE · PI BLUMBERG, BRUCE, SHIODA, TOSHIHIRO · 2020 to 2025
$2.4M
Roles of Nuclear Receptors in Generation of Heritable Epimutations in Germ CellsR21ES024861 · NIEHS · MASSACHUSETTS GENERAL HOSPITAL · PI SHIODA, TOSHIHIRO · 2015 to 2016
$471k
NIEHS NIH HHS R01 ES023316NIEHS NIH HHS R01 ES031139NIEHS NIH HHS R21 ES024861
6 · The paper itself

Abstract

Primordial germ cells (PGCs) are common ancestors of all germline cells. In mammals, PGCs emerge in early-stage embryos around the timing of gastrulation at or near epiblast, and specification of PGCs from their precursor cells involves multiple growth factors secreted by adjacent cells. Recent advancements in germline stem cell biology have made it possible to generate PGC-like cell culture models (PGCLCs for PGC-like cells) from human and mouse pluripotent stem cells by mimicking the embryonic growth factor environment in vitro. Here we describe a method of producing human PGCLCs from primed-pluripotency induced pluripotent stem cells (iPSCs) via temporal conversion to naive pluripotency followed by formation of embryoid bodies (EBs) using the spin-EB method.

Indexed as

Cell Culture TechniquesImmunohistochemistryImmunophenotypingBiomarkersCell DifferentiationCells, CulturedCell Self RenewalEmbryoid BodiesGastrulaGerm CellsGerm LayersHumansInduced Pluripotent Stem CellsBiomarkersAggreWellEmbryoid bodiesInduced pluripotent stem cellsNaive pluripotencyPrimed pluripotencyPrimordial germ cell–like cellsPrimordial germ cellsSpin-EB

Identifiers

PMID32852762
PMCPMC11804835
OpenAlexW3080973708

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.