ArticleJournal of assisted reproduction and genetics2020
Non-invasive imaging of mouse embryo metabolism in response to induced hypoxia.
Article in Journal of assisted reproduction and genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Evaluating Preimplantation Embryo Metabolism Through Imaging of Cellular Autofluorescence.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Investigating metabolic activity during oocyte and early embryo development through label-free metabolic imaging: a systematic approach for timelapse applications.Human reproduction (Oxford, England) · 2025Article
- Innovations in assisted reproductive technology through the advances in artificial intelligence and image processing.Obstetrics & gynecology science · 2025Review
- Micro magnetic resonance spectroscopy for noninvasive metabolic screening of mammalian embryos and oocytes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- METAPHOR: Metabolic evaluation through phasor-based hyperspectral imaging and organelle recognition for mouse blastocysts and oocytes.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Viewing early life without labels: optical approaches for imaging the early embryo†.Biology of reproduction · 2024Review
- Noninvasive metabolic profiling of cumulus cells, oocytes, and embryos via fluorescence lifetime imaging microscopy: a mini-review.Human reproduction (Oxford, England) · 2023Review
- Vitrification within a nanoliter volume: oocyte and embryo cryopreservation within a 3D photopolymerized device.Journal of assisted reproduction and genetics · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
purposeThis study used noninvasive, fluorescence lifetime imaging microscopy (FLIM)-based imaging of NADH and FAD to characterize the metabolic response of mouse embryos to short-term oxygen deprivation. We investigated the response to hypoxia at various preimplantation stages.
methodsMouse oocytes and embryos were exposed to transient hypoxia by dropping the oxygen concentration in media from 5-0% over the course of ~1.5 h, then 5% O
resultsMetabolic profiles showed significant changes in response to hypoxia for all stages of embryo development. The response of the eight measured FLIM parameters to hypoxia was highly stage-dependent. Of the eight FLIM parameters measured, NADH and FAD intensity showed the most dramatic metabolic responses in early developmental stages. At later stages, however, other parameters, such as NADH fraction engaged and FAD lifetimes, showed greater changes. Metabolic parameter values generally returned to baseline with the restoration of 5% oxygen.
conclusionsQuantitative FLIM-based metabolic imaging was highly sensitive to metabolic changes induced by hypoxia. Metabolic response profiles to oxygen deprivation were distinct at different stages, reflecting differences in metabolic plasticity as preimplantation embryos develop.
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Registered trials
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