ArticleBioTechniques2020
Antibody validation for protein expression on tissue slides: a protocol for immunohistochemistry.
Article in BioTechniques, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it.
- State-of-the-Art on Wound Vitality Evaluation: A Systematic Review.International journal of molecular sciences · 2022Pooled it
- Immunohistochemical expression of JAK2, STAT3, POLH, UBE2T, and REV7 in serous ovarian carcinoma: clinicopathological associations and co-expression patterns.Journal of molecular histology · 2026Article
- Resistance-exercise-induced stress intervenes in TGF-β signaling by cooperatively downregulating nuclear αB-crystallin and SMAD4 in human skeletal muscle fibers.The FEBS journal · 2026Article
- Histological features ofAnimal reproduction · 2026Article
- High-Plex Tissue Imaging with Conventional Immunofluorescence Platforms and Open-Source Software via Iterative Bleaching Extends Multiplexity (IBEX).Methods in molecular biology (Clifton, N.J.) · 2026Article
- Society for Immunotherapy of Cancer: Standards for Reporting of Multiplex Immunohistochemistry/Immunofluorescence Assays (STORMI).Journal for immunotherapy of cancer · 2025Article
- Validation and Prospective Testing of a High-Sensitivity, Quantitative Analytic Assay for HER2 on Histopathology Slides.Laboratory investigation; a journal of technical methods and pathology · 2025Article
- Evaluation of Glyoxal fixation for immunohistochemistry of the retina.Scientific reports · 2025Article
- Assessing expression patterns of PTGR1, a potential biomarker for acylfulven sensitivity in urothelial carcinoma.Scientific reports · 2024Article
- Quantitative Measurement of HER2 Expression in Non-Small Cell Lung Cancer With a High-Sensitivity Assay.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2024Article
- BCAM (basal cell adhesion molecule) protein expression in different tumor populations.Discover oncology · 2024Article
- Validation of an organ mapping antibody panel for cyclical immunofluorescence microscopy on normal human kidneys.American journal of physiology. Renal physiology · 2024Article
- Detecting Human Epidermal Growth Factor Receptor 2 (HER2) Amplification: Proof of Concept of an Alternative Approach.Cureus · 2023Article
- Spatial characterization and quantification of CD40 expression across cancer types.BMC cancer · 2023Article
- Quantitative, Spatially Defined Expression of Leukocyte-associated Immunoglobulin-like Receptor in Non-small Cell Lung Cancer.Cancer research communications · 2023Article
- In Situ Proximity Ligation Assay to Visualize Protein-Protein Interactions in Tumor Specimens.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Endothelial cell-specific mineralocorticoid receptor activation promotes diastolic dysfunction in diet-induced obese male mice.American journal of physiology. Regulatory, integrative and comparative physiology · 2023Article
- Digital spatial profiling of melanoma shows CD95 expression in immune cells is associated with resistance to immunotherapy.Oncoimmunology · 2023Article
- Immunohistochemical Detection of Estrogen Receptor-Beta (ERβ) with PPZ0506 Antibody in Murine Tissue: From Pitfalls to Optimization.Biomedicines · 2022Article
- Quantitative assessment of Siglec-15 expression in lung, breast, head, and neck squamous cell carcinoma and bladder cancer.Laboratory investigation; a journal of technical methods and pathology · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Antibodies play a crucial role in basic research and clinical decision-making. However, there are no standardized algorithms or guidelines to ensure their accuracy and validity. There have been efforts to generate consensus, but, with the exception of clinical labs, antibody validation remains variable in the literature and sometimes in clinical practice. Here we focus on immunohistochemistry, an example of a scientific and clinical tool where validation of antibodies is critical. We describe a protocol that we use to validate antibodies specifically for immunohistochemistry, including some of the pillars of antibody validation from Uhlen
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.