ArticleFrontiers in molecular biosciences2020
A Workflow for Protein Structure Determination From Thin Crystal Lamella by Micro-Electron Diffraction.
Article in Frontiers in molecular biosciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Strategies for mitigating radiation damage and improving data completeness in 3D electron diffraction of protein crystals.Acta crystallographica. Section D, Structural biology · 2026Article
- Review
- Cryo-tomography and 3D Electron Diffraction Reveal the Polar Habit and Chiral Structure of the Malaria Pigment Crystal Hemozoin.ACS central science · 2024Article
- TAAM refinement on high-resolution experimental and simulated 3D ED/MicroED data for organic molecules.Acta crystallographica. Section C, Structural chemistry · 2024Article
- Pillar data-acquisition strategies for cryo-electron tomography of beam-sensitive biological samples.Acta crystallographica. Section D, Structural biology · 2024Article
- Advances and applications of microcrystal electron diffraction (MicroED).Current opinion in structural biology · 2024Review
- Article
- A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals.Nature communications · 2023Article
- Electron Diffraction of 3D Molecular Crystals.Chemical reviews · 2022Review
- Ab initio phasing macromolecular structures using electron-counted MicroED data.Nature methods · 2022Article
- Review
- Benchmarking the ideal sample thickness in cryo-EM.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- 3D Electron Diffraction for Chemical Analysis: Instrumentation Developments and Innovative Applications.Chemical reviews · 2021Review
- Protocol for the use of focused ion-beam milling to prepare crystalline lamellae for microcrystal electron diffraction (MicroED).STAR protocols · 2021Article
- MicroED structure of the human adenosine receptor determined from a single nanocrystal in LCP.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A simple pressure-assisted method for MicroED specimen preparation.Nature communications · 2021Article
- Macromolecular crystallography using microcrystal electron diffraction.Acta crystallographica. Section D, Structural biology · 2021Review
- Small Molecule Microcrystal Electron Diffraction for the Pharmaceutical Industry-Lessons Learned From Examining Over Fifty Samples.Frontiers in molecular biosciences · 2021Article
- Statistically correcting dynamical electron scattering improves the refinement of protein nanocrystals, including charge refinement of coordinated metals.Acta crystallographica. Section D, Structural biology · 2021Article
- MicroED structure of lipid-embedded mammalian mitochondrial voltage-dependent anion channel.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
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10 authors.
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Abstract
MicroED has recently emerged as a powerful method for the analysis of biological structures at atomic resolution. This technique has been largely limited to protein nanocrystals which grow either as needles or plates measuring only a few hundred nanometers in thickness. Furthermore, traditional microED data processing uses established X-ray crystallography software that is not optimized for handling compound effects that are unique to electron diffraction data. Here, we present an integrated workflow for microED, from sample preparation by cryo-focused ion beam milling, through data collection with a standard Ceta-D detector, to data processing using the DIALS software suite, thus enabling routine atomic structure determination of protein crystals of any size and shape using microED. We demonstrate the effectiveness of the workflow by determining the structure of proteinase K to 2.0 Å resolution and show the advantage of using protein crystal lamellae over nanocrystals.
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