ArticleFrontiers in bioengineering and biotechnology2020
Bovine Milk Extracellular Vesicles Are Osteoprotective by Increasing Osteocyte Numbers and Targeting RANKL/OPG System in Experimental Models of Bone Loss.
Article in Frontiers in bioengineering and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Food-derived extracellular vesicles for treatment of osteoporosis: a systematic review and meta-analysis of preclinical animal studies.European journal of medical research · 2025Pooled it
- Role of the Antioxidant Lycopene in Bone Health: Emphasis on the RANKL/RANK/OPG Signaling Pathway.Food science & nutrition · 2026Review
- Bovine Milk-Derived Extracellular Vesicles as Emerging Drug Delivery Platforms: Current Advances, Applications and Challenges.Journal of extracellular biology · 2026Review
- Adherence to Chinese Dietary Guidelines Is Associated with Better Bone Status in School-Aged Children and Adolescents.Nutrients · 2026Article
- Milk-Derived EVs from Different Animal Sources: An Overview on Their Detection, Isolation and Pleiotropic Exerted Effects.International journal of molecular sciences · 2026Review
- Bovine Milk Extracellular Vesicles Modulate Alveolar Bone Microarchitecture and Mitigate Hepatic Steatosis in Obese Mice Fed a High-Fat Diet.Molecular nutrition & food research · 2026Article
- Non-Bone-Derived Extracellular Vesicles in Osteoporosis: Evidence Mapping and Nanomedicine Translation.International journal of nanomedicine · 2026Review
- Bovine Milk Extracellular Vesicles as a Preventive Treatment for Bone Dysfunction and Metabolic Alterations in Obese Mice Fed a High-Refined Carbohydrate Diet.Molecular nutrition & food research · 2025Article
- Collagen binding properties separate two functionally distinct subpopulations of milk extracellular vesicles regarding bone regenerative capacity.Materials today. Bio · 2025Article
- Harnessing Mammalian- and Plant-Derived Exosomes for Drug Delivery: A Comparative Review.International journal of molecular sciences · 2025Review
- Milk extracellular vesicles: A burgeoning new presence in nutraceuticals and drug delivery.Bioengineering & translational medicine · 2025Review
- The Role of Bovine Milk-Derived Exosomes in Human Health and Disease.Molecules (Basel, Switzerland) · 2024Review
- Article
- Therapeutic Potential of Bovine Milk-Derived Extracellular Vesicles.International journal of molecular sciences · 2024Review
- Genetic correlation and Mendelian randomization analyses support causal relationships between dietary habits and age at menarche.Scientific reports · 2024Article
- Orthodontic treatment during pregnancy, lactation, and postmenopausal period: a questionnaire development.Brazilian oral research · 2024Article
- Alternatives of mesenchymal stem cell-derived exosomes as potential therapeutic platforms.Frontiers in bioengineering and biotechnology · 2024Review
- Nutraceuticals in osteoporosis prevention.Frontiers in nutrition · 2024Review
- Extracellular Vesicles in Bone Remodeling and Osteoporosis.Advances in experimental medicine and biology · 2023Review
- Extracellular Vesicles from Animal Milk: Great Potentialities and Critical Issues.Animals : an open access journal from MDPI · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studying effects of milk components on bone may have a clinical impact as milk is highly associated with bone maintenance, and clinical studies provided controversial associations with dairy consumption. We aimed to evaluate the impact of milk extracellular vesicles (mEVs) on the dynamics of bone loss in mice. MEVs are nanoparticles containing proteins, mRNA and microRNA, and were supplemented into the drinking water of mice, either receiving diet-induced obesity or ovariectomy (OVX). Mice receiving mEVs were protected from the bone loss caused by diet-induced obesity. In a more severe model of bone loss, OVX, higher osteoclast numbers in the femur were found, which were lowered by mEV treatment. Additionally, the osteoclastogenic potential of bone marrow-derived precursor cells was lowered in mEV-treated mice. The reduced stiffness in the femur of OVX mice was consequently reversed by mEV treatment, accompanied by improvement in the bone microarchitecture. In general, the RANKL/OPG ratio increased systemically and locally in both models and was rescued by mEV treatment. The number of osteocytes, as primary regulators of the RANKL/OPG system, raised in the femur of the OVX mEVs-treated group compared to OVX non-treated mice. Also, the osteocyte cell line treated with mEVs demonstrated a lowered RANKL/OPG ratio. Thus, mEVs showed systemic and local osteoprotective properties in two mouse models of bone loss reflected in reduced osteoclast presence. Data reveal mEV potential in bone modulation, acting via osteocyte enhancement and RANKL/OPG regulation. We suggest that mEVs could be a therapeutic candidate for the treatment of bone loss.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.