ArticleFrontiers in bioengineering and biotechnology2020
3D-Printable and Enzymatically Active Composite Materials Based on Hydrogel-Filled High Internal Phase Emulsions.
Article in Frontiers in bioengineering and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it.
- High Internal Phase Pickering Emulsions as Structurally Tunable Bioinks for Extrusion-Based Printing From 3D Fabrication to Adaptive Multiresponsive Architectures.Comprehensive reviews in food science and food safety · 2026Pooled it
- Additive Manufacturing in Organic Chemistry: From Synthesis to Sustainable Process Design.International journal of molecular sciences · 2026Review
- Toward a Disruptive "Click-and-Run" 3D Printing Concept for Manufacturing Epidermal Wearable Electrochemical Sensors.ACS sensors · 2025Review
- Thiol-Acrylate polyHIPEs via Facile Layer-by-Layer Photopolymerization.3D printing and additive manufacturing · 2024Article
- Advances in 3D Gel Printing for Enzyme Immobilization.Gels (Basel, Switzerland) · 2022Review
- Emerging 3D Printing Strategies for Enzyme Immobilization: Materials, Methods, and Applications.ACS omega · 2022Review
- Systematic evaluation of agarose- and agar-based bioinks for extrusion-based bioprinting of enzymatically active hydrogels.Frontiers in bioengineering and biotechnology · 2022Article
- Virtual Reality as Tool for Bioprinting Quality Inspection: A Proof of Principle.Frontiers in bioengineering and biotechnology · 2022Article
- Considerations Using Additive Manufacture of Emulsion Inks to Produce Respiratory Protective Filters Against Viral Respiratory Tract Infections Such as the COVID-19 Virus.International journal of bioprinting · 2021Article
- Immobilization of β-Galactosidase by Encapsulation of Enzyme-Conjugated Polymer Nanoparticles Inside Hydrogel Microparticles.Frontiers in bioengineering and biotechnology · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The immobilization of enzymes in biocatalytic flow reactors is a common strategy to increase enzyme reusability and improve biocatalytic performance. Extrusion-based 3D bioprinting has recently emerged as a versatile tool for the fabrication of perfusable hydrogel grids containing entrapped enzymes for the use in such reactors. This study demonstrates the suitability of water-in-oil high internal phase emulsions (HIPEs) as 3D-printable bioinks for the fabrication of composite materials with a porous polymeric scaffold (polyHIPE) filled with enzyme-laden hydrogel. The prepared HIPEs exhibited excellent printability and are shown to be suitable for the printing of complex three-dimensional structures without the need for sacrificial support material. An automated activity assay method for the systematic screening of different material compositions in small-scale batch experiments is presented. The monomer mass fraction in the aqueous phase and the thickness of printed objects were found to be the most important parameters determining the apparent activity of the immobilized enzyme. Mass transfer limitations and enzyme inactivation were identified as probable factors reducing the apparent activity. The presented HIPE-based bioinks enable the fabrication of flow-optimized and more efficient biocatalytic reactors while the automated activity assay method allows the rapid screening of materials to optimize the biocatalytic efficiency further without time-consuming flow-through experiments involving whole printed reactors.
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