Evidence map›Paper›PMID 32849989›Full record

ArticleThe Pan African medical journal2020

Rapid detection of

Maaloum Fakhreddine, Katfy Khalid, Diraa Othman, Diawara Idrissa, Belabbes Houria, Zerouali Khalid

Open access · goldAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in The Pan African medical journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. [Summary of the clinical characteristics of tuberculous otitis media].Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Maaloum FakhreddineLaboratoire de Microbiologie, Faculté de Médecine et Pharmacie, Université Hassan II de Casablanca, Casablanca, Maroc.
Katfy KhalidLaboratoire de Microbiologie, Faculté de Médecine et Pharmacie, Université Hassan II de Casablanca, Casablanca, Maroc.
Diraa OthmanLaboratoire de Bactériologie, Virologie et Hygiène Hospitalière du CHU Ibn Rochd de Casablanca, Casablanca, Maroc.
Diawara IdrissaFaculté des Sciences et Techniques de Santé, Université Mohammed VI des Sciences de la Santé, Casablanca, Maroc.
Belabbes HouriaLaboratoire de Microbiologie, Faculté de Médecine et Pharmacie, Université Hassan II de Casablanca, Casablanca, Maroc.
Zerouali KhalidLaboratoire de Microbiologie, Faculté de Médecine et Pharmacie, Université Hassan II de Casablanca, Casablanca, Maroc.
University of Hassan II Casablanca · MACentre Hospitalier Universitaire Ibn Rochd · MAUniversité Mohammed VI des Sciences de la Santé · MA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionthe laboratory diagnosis of tuberculosis (TB) relies mainly on conventional techniques. However, it either lacks sensitivity or it is time-consuming. This study aims to evaluate the use of real-time polymerase chain reaction (PCR) targeting IS6110 for Mycobacterium tuberculosis (TB) Complex (MTBC) in the routine diagnosis of TB in our laboratory.

methodsclinical samples were collected from the laboratory of bacteriology at Ibn Rochd University Hospital in Casablanca Morocco. Real-time polymerase chain reaction (PCR) results were compared to AFB smear and culture on Löwenstein-Jensen (LJ) solid media. Sensitivity, specificity, positive and negative predictive value (PPV and NPV) with 95% confidence intervals were calculated using GraphPad Prism.

resultson 171 clinical samples, the study showed positivity of microscopy, culture and real-time PCR for M. TB complex as 19%, 31%, and 32% respectively. Sensitivity, specificity, PPV and NPV for real-time PCR in pulmonary samples were 95.2%, 95.4%, 90.91% and 97.65% respectively. For extra-pulmonary samples, they were: 72.7%, 90.32%, 72.7%, and 90.3%.

conclusionour study shows the effectiveness of using real-time PCR IS6110 in pulmonary and extra pulmonary samples. Future multicentric studies could seek to evaluate the place of this technique on routine diagnosis for better management of TB in Morocco.

Indexed as

Antigens, BacterialDiagnostic Tests, RoutineHumansMicrobiological TechniquesMoroccoMycobacterium tuberculosisPredictive Value of TestsReal-Time Polymerase Chain ReactionSensitivity and SpecificitySpecimen HandlingSputumTuberculosisTuberculosis, PulmonaryAntigens, BacterialIS6110Mycobacterium tuberculosis complexreal-time polymerase chain reactiontuberculosis

Identifiers

PMID32849989
PMCPMC7422747
OpenAlexW3037145398

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.