ArticleFrontiers in plant science2020
Vibratome Sectioning and Clearing for Easing Studies of Cassava Embryo Formation.
Article in Frontiers in plant science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- A Method for Detecting Parthenogenesis in Asteraceae Species.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Fifty years of a public cassava breeding program: evolution of breeding objectives, methods, and decision-making processes.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Review
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3 authors.
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Abstract
This work describes the application of clearing on vibratome sections to study the embryo formation in cassava. This procedure provides high-resolution images and reduces significantly the number of sections that need to be analyzed per ovule. This methodology was instrumental for the development of the protocol for embryo rescue in cassava. It has been also applied to monitor the embryo formation response when optimizing seed setting from regular and broad crosses for cassava breeding. Broad crosses between cassava and castor bean (incompatible-euphorbiaceae species) were made aiming to induce doubled haploids through the elimination of the incompatible-male parent genome as done in cereals. Castor bean is widely available and provides continues supply of pollen. Our results suggest that this methodology is easy and effective to assess the response of hundreds of cassava ovules pollinated with castor bean pollen, allowing the identification of multicellular structures in the embryo sac without apparent formation of endosperm. The protocol is also useful when developing and optimizing a methodology to induce doubled haploids in cassava
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