Evidence map›Paper›PMID 32849615›Full record

ArticleFrontiers in immunology2020

Differently Expression Analysis and Function Prediction of Long Non-coding RNAs in Duck Embryo Fibroblast Cells Infected by Duck Tembusu Virus.

Yun Lin, Jing Yang, Dalin He, Xudong Li, Jing Li, Yi Tang, Youxiang Diao

Open access · goldAbstract read
In one paragraph

Article in Frontiers in immunology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
3.6field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Effect ofNutrients · 2022
    Article
  5. Article
  6. LncRNAs in domesticated animals: from dog to livestock species.Mammalian genome : official journal of the International Mammalian Genome Society · 2022
    Review
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Yun LinCollege of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
Jing YangCollege of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
Dalin HeCollege of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
Xudong LiCollege of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
Jing LiCollege of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
Yi TangCollege of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
Youxiang DiaoCollege of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
Shandong Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Duck Tembusu virus (DTMUV), the causative agent of egg-drop syndrome, has caused substantial economic losses to duck industry. DTMUV infection leads to profound changes of host cells, including transcriptome and proteome. However, the lncRNA expression profile and the biological function of lncRNA have not been revealed. Therefore, DTMUV was used to inoculate duck embryo fibroblast cells (DEFs) for high-throughput RNA-sequencing (RNA-Seq). The results showed that 34 and 339 differently expressed lncRNAs were, respectively, identified at 12 and 24 h post-infection (hpi). To analyze their biological functions, target genes in cis were searched and the regulatory network was formed. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that the target genes were strongly associated with immune system, signaling molecular and interaction, endocrine system, and signal transduction. The differently expressed lncRNAs were selected and verified by quantitative real-time polymerase chain reaction (RT-qPCR). Our study, for the first time, analyzed a comprehensive lncRNA expression profile in DEFs following DTMUV infection. The analysis provided a view on the important roles of lncRNAs in gene regulation and DTMUV infection.

Indexed as

TranscriptomeAnimalsCells, CulturedDucksFibroblastsFlavivirusFlavivirus InfectionsGene Expression ProfilingGene Regulatory NetworksHigh-Throughput Nucleotide SequencingHost-Pathogen InteractionsPoultry DiseasesReal-Time Polymerase Chain ReactionRNA, Long NoncodingRNA-SeqRNA, Long NoncodingDEFsDTMUV infectiongene regulationlncRNA expressionRNA-Seq

Identifiers

PMID32849615
PMCPMC7417515
OpenAlexW3047259961

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.