ArticleFrontiers in microbiology2020
Optimization of Viability Treatment Essential for Accurate Droplet Digital PCR Enumeration of Probiotics.
Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- Optimized Viability-ddPCR with Triton X-100 Enhancement for Selective Detection of LiveJournal of microbiology and biotechnology · 2026Article
- Factors affecting the viability of probiotics in various food products: a review of current knowledge.Frontiers in microbiology · 2026Review
- Postbiotics: a perspective on their quantification.Frontiers in nutrition · 2025Article
- Nanobodies as potential tools for microbiological testing of live biotherapeutic products.AMB Express · 2024Article
- Real-time PCR methods for identification and stability monitoring ofFrontiers in microbiology · 2024Article
- The Effect of Delivery Matrix onNutrients · 2023Article
- Probiotic and postbiotic analytical methods: a perspective of available enumeration techniques.Frontiers in microbiology · 2023Review
- Digital PCR Applications in the SARS-CoV-2/COVID-19 Era: a Roadmap for Future Outbreaks.Clinical microbiology reviews · 2022Review
- Changes in physiological states of Salmonella Typhimurium measured by qPCR with PMA and DyeTox13 Green Azide after pasteurization and UV treatment.Applied microbiology and biotechnology · 2022Article
- Viability droplet digital polymerase chain reaction accurately enumerates probiotics and provides insight into damage experienced during storage.Frontiers in microbiology · 2022Article
- An Efficient Tetraplex Surveillance Tool for Salmonid Pathogens.Frontiers in microbiology · 2022Article
- Real-time polymerase chain reaction methods for strain specific identification and enumeration of strainFrontiers in microbiology · 2022Article
- Enumeration of Probiotic Strain Lacticaseibacillus rhamnosus GG (ATCC 53103) Using Viability Real-time PCR.Probiotics and antimicrobial proteins · 2021Article
- Assessing Viability and Stress Tolerance of Probiotics-A Review.Frontiers in microbiology · 2021Review
- Locked Nucleic Acid Hydrolysis Probes for the Specific Identification of Probiotic StrainsFrontiers in microbiology · 2021Article
- The Development of High-Quality Multispecies Probiotic Formulations: From Bench to Market.Nutrients · 2020Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Improvements offered by viability droplet digital PCR (v-ddPCR) include increased precision, specificity and decreased time to results making for an attractive alternative method to traditional plate count enumeration of probiotic products. A major hurdle faced in v-ddPCR, however, is distinguishing between live and dead cells. The objective of this study was to evaluate a combination of PMA and EMA (PE51) for viability treatment of freeze-dried probiotic powders.
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Registered trials
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