ArticleFrontiers in microbiology2020
Metagenomic Characterization of Indoor Dust Bacterial and Fungal Microbiota in Homes of Asthma and Non-asthma Patients Using Next Generation Sequencing.
Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Revised ISHAM-ABPA working group clinical practice guidelines for diagnosing, classifying and treating allergic bronchopulmonary aspergillosis/mycoses.The European respiratory journal · 2024Guideline
- Correlation BetweenJournal of fungi (Basel, Switzerland) · 2025Article
- Environmental and structural factors associated with bacterial diversity in household dust across the Arizona-Sonora border.Scientific reports · 2024Article
- Method for detection of pathogenic bacteria from indoor air microbiome samples using high-throughput amplicon sequencing.MethodsX · 2024Article
- The Role of Biodiversity in the Development of Asthma and Allergic Sensitization: A State-of-the-Science Review.Environmental health perspectives · 2024Review
- Fungal Colonization of the Airways of Patients with Cystic Fibrosis: the Role of the Environmental Reservoirs.Mycopathologia · 2024Review
- Environmental and Structural Factors Associated with Bacterial Diversity in Household Dust Across the Arizona-Sonora Border.Research square · 2023Article
- Multi-omics reveal microbial determinants impacting the treatment outcome of antidepressants in major depressive disorder.Microbiome · 2023Article
- A comprehensive review of microbial contamination in the indoor environment: sources, sampling, health risks, and mitigation strategies.Frontiers in public health · 2023Review
- Review
- Air Sampling for Fungus around Hospitalized Patients with Coronavirus Disease 2019.Journal of fungi (Basel, Switzerland) · 2022Article
- Microplastics exposure causes oxidative stress and microbiota dysbiosis in planarian Dugesia japonica.Environmental science and pollution research international · 2022Article
- Determinants of bacterial and fungal microbiota in Finnish home dust: Impact of environmental biodiversity, pets, and occupants.Frontiers in microbiology · 2022Article
- A Pilot Study on Baseline Fungi and Moisture Indicator Fungi in Danish Homes.Journal of fungi (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe exposure of house occupants to indoor air pollutants has increased in recent decades. Among microbiological contaminants, bacterial and fungal aerosols remain poorly studied and the debate on the impact of these aerosols on respiratory health is still open. This study aimed to assess the diversity of indoor microbial communities in relationship with the health of occupants.
methodsMeasurements were taken from dwellings of 2 cohorts in Brittany (France), one with children without any pathology and the other with children and adults with asthma. Thirty dust samples were analyzed by next generation sequencing with a 16S and 18S targeted metagenomics approach. Analysis of sequencing data was performed using qiime 2, and univariate and multivariate statistical analysis using R software and phyloseq package.
resultsA total of 2,637 prokaryotic (589 at genus level) and 2,153 eukaryotic taxa were identified (856 fungal taxa (39%) and 573 metazoa (26%)). The four main bacterial phyla were identified: Proteobacteria (53%), Firmicutes (27%), Actinobacteria (11%), Bacteroidetes (8%). Among Fungi, only 136 taxa were identified at genus level. Three main fungal phyla were identified: Ascomycota (84%), Basidiomycota (12%) and Mucoromycota (3%). No bacterial nor fungal phyla were significantly associated with asthma versus control group. A significant over representation in control group versus asthma was observed for Christensenellaceae family (
conclusionsOur findings provide evidence that dust samples harbor a high diversity of human-associated bacteria and fungi. Molecular methods such as next generation sequencing are reliable tools for identifying and tracking the bacterial and fungal diversity in dust samples, a less easy strategy for the detection of eukaryotes at least using18S metagenomics approach. This study showed that the detection of some bacteria might be associated to indoor air of asthmatic patients. Regarding fungi, a higher number of samples and sequencing with more depth could allow reaching significant signatures.
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