ArticleVeterinary world2020
Molecular characterization of the 2018 outbreak of lumpy skin disease in cattle in Upper Egypt.
Article in Veterinary world, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Global expansion of lumpy skin disease: transmission trends, economic consequences, and preventive measures.Veterinary research communications · 2025Review
- Progress in diagnostic methods and vaccines for lumpy skin disease virus: a path towards understanding the disease.Veterinary research communications · 2025Review
- Geographical Distribution, Spatial Directional Trends, and Spatio-Temporal Clusters of the First Rapid and Widespread Lumpy Skin Disease Outbreaks in Thailand.Transboundary and emerging diseases · 2025Article
- Promising antiviral inhibitors against lumpy skin disease: A vetinformatics approach.Open veterinary journal · 2024Article
- Geospatial Analysis of Lumpy Skin Disease Outbreaks among Cattle in Uttar Pradesh, India, 2021-2022.Pathogens (Basel, Switzerland) · 2024Article
- Space-Time Clustering and Climatic Risk Factors for Lumpy Skin Disease of Cattle in Uttar Pradesh, India, 2022.Transboundary and emerging diseases · 2024Article
- Emergency vaccination of cattle against lumpy skin disease: Evaluation of safety, efficacy, and potency of MEVACVeterinary research communications · 2023Article
- Article
- Lumpy Skin Disease Outbreaks in Africa, Europe, and Asia (2005-2022): Multiple Change Point Analysis and Time Series Forecast.Viruses · 2022Article
- Isolation and molecular characterization of lumpy skin disease virus from hard ticks, Rhipicephalus (Boophilus) annulatus in Egypt.BMC veterinary research · 2022Article
- Spatio-temporal patterns of lumpy skin disease outbreaks in dairy farms in northeastern Thailand.Frontiers in veterinary science · 2022Article
- Molecular diagnosis of three outbreaks during three successive years (2018, 2019, and 2020) ofOpen veterinary journalArticle
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimLumpy skin disease (LSD), an infectious disease of cattle, is characterized by raised nodules on the skin. Although the morbidity rate of LSD is low, it has a considerable fatality rate. Despite the annual mass vaccination of livestock with sheep pox vaccine (Veterinary Serum and Vaccine Research Institute, Egypt) enforced by Egyptian authorities, the LSD virus (LSDV) continues to circulate almost every summer. The present study aimed to discover the cause of cows naturally infected with LSDV circulating in Upper Egypt during the summer of 2018 using polymerase chain reaction (PCR) assay and to analyze their phylogenetics against reference genome sequences. MATERIALS AND
methodsWe cultured LSDV in specific pathogen-free embryonated chicken eggs (SPF-ECE) and used conventional PCR to identify fusion and P32 genes, previously deposited in GenBank (MN694826, MN694827, and MN954664). Sequencing and phylogenetic analyses were performed on these two highly conserved viral genes.
resultsLSDV infection of SPF-ECE resulted in characteristic white pock lesions. PCR products were identified on 1.5% agarose gel after electrophoresis at the expected positions for the fusion and P32 genes at 472 and 587 bp, respectively.
conclusionThe present study revealed that the two viral genes were identified from the Beni Suef and Sohag Governorates in all clinical cases and confirmed the circulation of LSDV in this outbreak. After sequencing, these genes were identical to those of the LSDV that had been identified and recorded in GenBank for the past 3 years.
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