ArticleBMC microbiology2020
Detection of clinically important non tuberculous mycobacteria (NTM) from pulmonary samples through one-step multiplex PCR assay.
Article in BMC microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- One-step multiplex PCR assay for identification ofMicrobiology spectrum · 2026Article
- Diagnostic Accuracy of AdvanSureDiagnostics (Basel, Switzerland) · 2025Article
- Simultaneous detection and differentiation ofMicrobiology spectrum · 2025Article
- Article
- From anonymity to stardom: history of nontuberculous mycobacterial disease in humans.Frontiers in cellular and infection microbiology · 2025Review
- Identification of Nontuberculous Mycobacteria in Patients with Multidrug-resistant Tuberculosis in Quezon City, Philippines, Using Multiplex PCR.Acta medica Philippina · 2025Article
- Review
- Application of a new designed high resolution melting analysis for mycobacterial species identification.BMC microbiology · 2024Article
- First Case Reports of Nontuberculous Mycobacterial (NTM) Lung Disease in Ecuador: Important Lessons to Learn.Pathogens (Basel, Switzerland) · 2023Article
- Characteristics of clinical isolates of nontuberculous mycobacteria in Java-Indonesia: A multicenter study.PLoS neglected tropical diseases · 2022Article
- Identification of Nontuberculous Mycobacteria in Patients with Pulmonary Diseases in Gyeongnam, Korea, Using Multiplex PCR and Multigene Sequence-Based Analysis.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe burden of non-tuberculous mycobacterial (NTM) disease is increasing worldwide but still its diagnosis is delayed and it is mistaken as multidrug-resistant tuberculosis (MDR-TB).The present study was performed to develop a multiplex PCR assay for detection and identification of clinically most common NTM to the species level from pulmonary samples.
resultsOut of 50 isolates, 26 were identified as Mycobacterium kansasii (MK), 20 were identified as Mycobacterium abscessus (MA) and 4 were identified as Mycobacterium avium complex (MAC) through multiplex PCR and further confirmed by sequencing.
conclusionOur study showed that multiplex PCR assay is a simple, convenient, and reliable technique for detection and differential identification of major NTM species.
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