ArticleCell2020
Thermus thermophilus Argonaute Functions in the Completion of DNA Replication.
Article in Cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
60 citing papers in PubMed, 126 citations in OpenAlex.
- A DNA-guided prokaryotic argonaute enables programmable RNA cleavage for sequencing and quality control ofMolecular therapy. Nucleic acids · 2026Article
- Review
- Moderate-temperature DNA cleavage activity of TtAgo activated by dCTP/TthSSB for one-step isothermal microRNAs detection.Nature communications · 2026Article
- A Programmable Eukaryotic Argonaute Nuclease with Dual DNA and RNA Cleavage Activity from Thermophilic FungusSmall science · 2026Article
- SSB-mediated enhancement of argonaute activity triggers SOS filamentation in bacteria.Nucleic acids research · 2026Article
- Specificity of DNA targeting by RNA-guided pAgo nucleases.Nucleic acids research · 2026Article
- Activation of the SPARDA defense system by filament assembly using a beta-relay signaling mechanism widespread in prokaryotic Argonautes.Cell research · 2025Article
- A programmable pAgo nuclease with high activity and specificity for efficient DNA and RNA manipulation.Communications biology · 2025Article
- Transcription-replication conflict resolution by nuclear RNA interference.Molecular cell · 2025Article
- Structural and evolutionary determinants of Argonaute function.Nucleic acids research · 2025Article
- Cyanobacterial Argonautes and Cas4 family nucleases cooperate to interfere with invading DNA.Molecular cell · 2025Article
- Excessive Existence of Positively Charged Amino Acids Caused Off-Target Recognition in the Seed Region ofInternational journal of molecular sciences · 2025Article
- The role of prokaryotic argonautes in resistance to type II topoisomerases poison ciprofloxacin.Biochemical Society transactions · 2024Review
- Structural and mechanistic insights into a mesophilic prokaryotic Argonaute.Nucleic acids research · 2024Article
- RNA-guided RNA silencing by an Asgard archaeal Argonaute.Nature communications · 2024Article
- The clade-specific target recognition mechanisms of plant RISCs.Nucleic acids research · 2024Article
- Prokaryotic Argonaute nuclease cooperates with co-encoded RNase to acquire guide RNAs and target invader DNA.Nucleic acids research · 2024Article
- Differential requirement for RecFOR pathway components in Thermus thermophilus.Environmental microbiology reports · 2024Article
- DNA-targeting short Argonautes complex with effector proteins for collateral nuclease activity and bacterial population immunity.Nature microbiology · 2024Article
- Target DNA-dependent activation mechanism of the prokaryotic immune system SPARTA.Nucleic acids research · 2024Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
In many eukaryotes, Argonaute proteins, guided by short RNA sequences, defend cells against transposons and viruses. In the eubacterium Thermus thermophilus, the DNA-guided Argonaute TtAgo defends against transformation by DNA plasmids. Here, we report that TtAgo also participates in DNA replication. In vivo, TtAgo binds 15- to 18-nt DNA guides derived from the chromosomal region where replication terminates and associates with proteins known to act in DNA replication. When gyrase, the sole T. thermophilus type II topoisomerase, is inhibited, TtAgo allows the bacterium to finish replicating its circular genome. In contrast, loss of gyrase and TtAgo activity slows growth and produces long sausage-like filaments in which the individual bacteria are linked by DNA. Finally, wild-type T. thermophilus outcompetes an otherwise isogenic strain lacking TtAgo. We propose that the primary role of TtAgo is to help T. thermophilus disentangle the catenated circular chromosomes generated by DNA replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.