ArticlePLoS genetics2020
Uptake of exogenous serine is important to maintain sphingolipid homeostasis in Saccharomyces cerevisiae.
Article in PLoS genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Engineering serine metabolism to enhance AOX1 promoter self-induction in formate dehydrogenase-deficient Komagataella phaffii.Microbial cell factories · 2026Article
- DEAD-box ATPase-marked condensates coordinate compartmentalized translation and antibiotic persistence.Science advances · 2026Article
- Sphingolipid and methionine metabolism in aging.Journal of cell science · 2025Review
- Structure of the yeast ceramide synthase.Nature structural & molecular biology · 2025Article
- Auxotrophy-based curation improves the consensus genome-scale metabolic model of yeast.Synthetic and systems biotechnology · 2024Article
- Antifungal Activity of Disalt of Epipyrone A fromJournal of fungi (Basel, Switzerland) · 2024Article
- Different Nitrogen Consumption Patterns in Low Temperature Fermentations in the Wine YeastFoods (Basel, Switzerland) · 2024Article
- Serine Rejuvenated DegeneratedJournal of fungi (Basel, Switzerland) · 2024Article
- Neuroinflammation underlies the development of social stress induced cognitive deficit in male sickle cell mice.Experimental biology and medicine (Maywood, N.J.) · 2024Article
- Structure of the ceramide-bound SPOTS complex.Nature communications · 2023Article
- Biochemical and metabolomic insights into antifungal mechanism of berberine against Candida glabrata.Applied microbiology and biotechnology · 2023Article
- Yeast Svf1 binds ceramides and contributes to sphingolipid metabolism at the ER cis-Golgi interface.The Journal of cell biology · 2023Article
- Cysteine restriction-specific effects of sulfur amino acid restriction on lipid metabolism.Aging cell · 2022Article
- Eisosome disruption by noncoding RNA deletion increases protein secretion in yeast.PNAS nexus · 2022Article
- A Light-Activatable Photocaged Variant of the Ultra-High Affinity ALFA-Tag Nanobody.Chembiochem : a European journal of chemical biology · 2022Article
- Article
- Review
- Review
- Autophagy promotes mitochondrial respiration by providing serine for one-carbon-metabolism.Autophagy · 2021Article
- Regulation of sphingolipid synthesis by the G1/S transcription factor Swi4.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sphingolipids are abundant and essential molecules in eukaryotes that have crucial functions as signaling molecules and as membrane components. Sphingolipid biosynthesis starts in the endoplasmic reticulum with the condensation of serine and palmitoyl-CoA. Sphingolipid biosynthesis is highly regulated to maintain sphingolipid homeostasis. Even though, serine is an essential component of the sphingolipid biosynthesis pathway, its role in maintaining sphingolipid homeostasis has not been precisely studied. Here we show that serine uptake is an important factor for the regulation of sphingolipid biosynthesis in Saccharomyces cerevisiae. Using genetic experiments, we find the broad-specificity amino acid permease Gnp1 to be important for serine uptake. We confirm these results with serine uptake assays in gnp1Δ cells. We further show that uptake of exogenous serine by Gnp1 is important to maintain cellular serine levels and observe a specific connection between serine uptake and the first step of sphingolipid biosynthesis. Using mass spectrometry-based flux analysis, we further observed imported serine as the main source for de novo sphingolipid biosynthesis. Our results demonstrate that yeast cells preferentially use the uptake of exogenous serine to regulate sphingolipid biosynthesis. Our study can also be a starting point to analyze the role of serine uptake in mammalian sphingolipid metabolism.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.