ArticleACS nano2020
Quantum Dot-Conjugated SARS-CoV-2 Spike Pseudo-Virions Enable Tracking of Angiotensin Converting Enzyme 2 Binding and Endocytosis.
Article in ACS nano, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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Who cites it
58 citing papers in PubMed, 116 citations in OpenAlex.
- Nonenzymatic Detection of SARS-CoV‑2 RNA Using DNA Nanoswitches.ACS nano medicine · 2026Article
- Size-Dependent Neutralization Efficacy of Nanodecoys Against SARS-CoV-2 Mimics in Mammalian Cell Infection Models.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Uptake of Nanoparticles as a Model System for Viruses under the Presence of Chloroquine.ACS nanoscience Au · 2026Article
- Advanced fluorescence biosensor utilizing nitrogen/oxygen-doped carbon quantum dots in a modified carboxy cellulose matrix for quantitative detection of SARS-CoV-2 spike protein.Discover nano · 2026Article
- Receptor Binding for the Entry Mechanisms of SARS-CoV-2: Insights from the Original Strain and Emerging Variants.Viruses · 2025Review
- Imaging of Viral Genomic RNA Replication with Nanoprobes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Article
- SLC38A9 regulates SARS-CoV-2 viral entry.iScience · 2024Article
- On-site airborne pathogen detection for infection risk mitigation.Chemical Society reviews · 2023Review
- Recent Updates on Multifunctional Nanomaterials as Antipathogens in Humans and Livestock: Classification, Application, Mode of Action, and Challenges.Molecules (Basel, Switzerland) · 2023Review
- Screening Drugs for Broad-Spectrum, Host-Directed Antiviral Activity: Lessons from the Development of Probenecid for COVID-19.Viruses · 2023Article
- Single virus fingerprinting by widefield interferometric defocus-enhanced mid-infrared photothermal microscopy.Nature communications · 2023Article
- Quantum Dot Biomimetic for SARS-CoV-2 to Interrogate Blood-Brain Barrier Damage Relevant to NeuroCOVID Brain Inflammation.ACS applied nano materials · 2023Article
- Recent Advances in Quantum Dot-Based Lateral Flow Immunoassays for the Rapid, Point-of-Care Diagnosis of COVID-19.Biosensors · 2023Review
- Identification of nanoparticles as vesicular cargoNanoscale advances · 2023Article
- Nanotechnology in the COVID-19 era: Carbon-based nanomaterials as a promising solution.Carbon · 2023Review
- Confocal interferometric scattering microscopy reveals 3D nanoscopic structure and dynamics in live cells.Nature communications · 2023Article
- SARS-CoV-2 replication in airway epithelia requires motile cilia and microvillar reprogramming.Cell · 2023Article
- Pseudotyped Viruses for Coronaviruses.Advances in experimental medicine and biology · 2023Article
- SARS-CoV-2 Spike Protein (RBD) Subunit Adsorption at Abiotic Surfaces and Corona Formation at Polymer Particles.International journal of molecular sciences · 2022Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The first step of SARS-CoV-2 infection is binding of the spike protein's receptor binding domain to the host cell's ACE2 receptor on the plasma membrane. Here, we have generated a versatile imaging probe using recombinant Spike receptor binding domain conjugated to fluorescent quantum dots (QDs). This probe is capable of engaging in energy transfer quenching with ACE2-conjugated gold nanoparticles to enable monitoring of the binding event in solution. Neutralizing antibodies and recombinant human ACE2 blocked quenching, demonstrating a specific binding interaction. In cells transfected with ACE2-GFP, we observed immediate binding of the probe on the cell surface followed by endocytosis. Neutralizing antibodies and ACE2-Fc fully prevented binding and endocytosis with low nanomolar potency. Importantly, we will be able to use this QD nanoparticle probe to identify and validate inhibitors of the SARS-CoV-2 Spike and ACE2 receptor binding in human cells. This work enables facile, rapid, and high-throughput cell-based screening of inhibitors for coronavirus Spike-mediated cell recognition and entry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.