Evidence map›Paper›PMID 32843919›Full record

ArticleWorld journal of stem cells2020

Bone marrow mesenchymal stem cells induce M2 microglia polarization through PDGF-AA/MANF signaling.

Fan Yang, Wen-Bin Li, Ye-Wei Qu, Jin-Xing Gao, Yu-Shi Tang, Dong-Jie Wang, Yu-Jun Pan

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of stem cells, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 53 citations in OpenAlex.

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  8. Inflammaging and Brain Aging.International journal of molecular sciences · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Fan YangDepartment of Neurology, The First Clinical College of Harbin Medical University, Harbin 150001, Heilongjiang Province, China.
Wen-Bin LiDepartment of Neurology, The First Clinical College of Harbin Medical University, Harbin 150001, Heilongjiang Province, China.
Ye-Wei QuDepartment of Neurology, The First Clinical College of Harbin Medical University, Harbin 150001, Heilongjiang Province, China.
Jin-Xing GaoDepartment of Neurology, The First Clinical College of Harbin Medical University, Harbin 150001, Heilongjiang Province, China.
Yu-Shi TangDepartment of Neurology, The First Clinical College of Harbin Medical University, Harbin 150001, Heilongjiang Province, China.
Dong-Jie WangDepartment of Respiratory Medicine, The First Clinical College of Harbin Medical University, Harbin 150001, Heilongjiang Province, China.
Yu-Jun PanDepartment of Neurology, The First Clinical College of Harbin Medical University, Harbin 150001, Heilongjiang Province, China. yujunpan@ems.hrbmu.edu.cn.
Harbin Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBone marrow mesenchymal stem cells (BMSCs) are capable of shifting the microglia/macrophages phenotype from M1 to M2, contributing to BMSCs-induced brain repair. However, the regulatory mechanism of BMSCs on microglia/macrophages after ischemic stroke is unclear. Recent evidence suggests that mesencephalic astrocyte-derived neurotrophic factor (MANF) and platelet-derived growth factor-AA (PDGF-AA)/MANF signaling regulate M1/M2 macrophage polarization.

aimTo investigate whether and how MANF or PDGF-AA/MANF signaling influences BMSCs-mediated M2 polarization.

methodsWe identified the secretion of MANF by BMSCs and developed transgenic BMSCs using a targeting small interfering RNA for knockdown of MANF expression. Using a rat middle cerebral artery occlusion (MCAO) model transplanted by BMSCs and BMSCs-microglia Transwell coculture system, the effect of BMSCs-induced downregulation of MANF expression on the phenotype of microglia/macrophages was tested by Western blot, quantitative reverse transcription-polymerase chain reaction, and immunofluorescence. Additionally, microglia were transfected with mimics of miR-30a*, which influenced expression of X-box binding protein (XBP) 1, a key transcription factor that synergized with activating transcription factor 6 (ATF6) to govern MANF expression. We examined the levels of miR-30a*, ATF6, XBP1, and MANF after PDGF-AA treatment in the activated microglia.

resultsInhibition of MANF attenuated BMSCs-induced functional recovery and decreased M2 marker production, but increased M1 marker expression

conclusionBMSCs-mediated MANF paracrine signaling, in particular the PDGF-AA/miR-30a*/XBP1/MANF pathway, synergistically mediates BMSCs-induced M2 polarization.

Indexed as

Bone marrow mesenchymal stem cellCerebral ischemia/reperfusion injuryEndoplasmic reticulum stressMesencephalic astrocyte–derived neurotrophic factorMicroglia/macrophage polarization

Identifiers

PMID32843919
PMCPMC7415242
OpenAlexW3045361000

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.