ArticleScientific reports2020
The proanthocyanin-related transcription factors MYBC1 and WRKY44 regulate branch points in the kiwifruit anthocyanin pathway.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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49 citing papers in PubMed, 92 citations in OpenAlex.
- Metabolomic variation associated with coloration in purple-fleshed sweet potato storage roots under different substrate conditions.Food chemistry: X · 2026Article
- WRKY14-DPB Module Enhances Drought Tolerance by Activating the Expression of UGT84B1 Involved in Hydrolyzable Tannin Biosynthesis.Plant, cell & environment · 2026Article
- Anthocyanin accumulation in fruit crops: a multi-layered regulatory network integrating molecular, epigenetic, hormonal and environmental regulation.Molecular biology reports · 2026Review
- Two LEAFY homologs regulate floral patterning and development without affecting flowering time in kiwifruit.Journal of experimental botany · 2026Article
- TheFood chemistry. Molecular sciences · 2026Article
- Integrated metabolome and transcriptome analyses reveal the role played by CaWRKY44 during anthocyanins accumulation in pepper fruits.BMC plant biology · 2026Article
- AaMYB114 promotes the coloration of Actinidia arguta by synthesizing and transporting anthocyanins.BMC plant biology · 2026Article
- Molecular insights into the regulation of flavonoid biosynthesis in fruits.Horticulture research · 2026Article
- The RcMYB308-RcEGL1 module regulates cyanidin accumulation by targeting the RcF3'H promoter in pink roses.BMC plant biology · 2026Article
- Mechanisms of acclimation regulation and stress response to UV-B inFrontiers in plant science · 2026Article
- Article
- Overexpression ofPlants (Basel, Switzerland) · 2025Article
- An R2R3 MYB transcription factor GhMYB5: regulator of CHS expression and proanthocyanin synthesis in brown cotton (Gossypium hirsutum L.).BMC genomics · 2025Article
- Combination of 3D chromatin architecture and omics analysis provides insight into anthocyanin regulation inHorticulture research · 2025Article
- RsWRKY44 participated in anthocyanin biosynthesis regulation in radish through interaction with RsMYB1a.Plant cell reports · 2025Article
- Chromosome-level genome assembly assisting for dissecting mechanism of anthocyanin regulation in kiwifruit (Actinidia arguta).Molecular horticulture · 2025Article
- Stable plastid transformation in kiwifruit (aBIOTECH · 2025Article
- AaMYB61-like and AabHLH137 jointly regulate anthocyanin biosynthesis in Actinidia arguta.BMC plant biology · 2025Article
- Comprehensive analysis of metabolomics and transcriptomics reveals varied tepal pigmentation across Gloriosa varieties.BMC plant biology · 2025Article
- Integrated metabolomics and transcriptomics unravel the biosynthZaesis mechanism of anthocyanin in postharvest red raspberry (Frontiers in plant science · 2025Article
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Authors and funding
6 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The groups of plant flavonoid metabolites termed anthocyanins and proanthocyanins (PA) are responsible for pigmentation in seeds, flowers and fruits. Anthocyanins and PAs are produced by a pathway of enzymes which are transcriptionally regulated by transcription factors (TFs) that form the MYB-bHLH-WD40 (MBW) complex. In this study, transcriptomic analysis of purple-pigmented kiwifruit skin and flesh tissues identified MYBC1, from subgroup 5 of the R2R3 MYB family, and WRKY44 (highly similar to Arabidopsis TTG2) as candidate activators of the anthocyanin pathway. Transient over-expression of MYBC1 and WRKY44 induced anthocyanin accumulation in tobacco leaves. Dual luciferase promoter activation assays revealed that both MYBC1 and WRKY44 were able to strongly activate the promoters of the kiwifruit F3'H and F3'5'H genes. These enzymes are branch points of the pathway which specifies the type of anthocyanin accumulated. Stable over-expression of MYBC1 and WRKY44 in kiwifruit calli activated the expression of F3'5'H and PA-related biosynthetic genes as well as increasing levels of PAs. These results suggest that while previously characterised anthocyanin activator MYBs regulate the overall anthocyanin biosynthesis pathway, the PA-related TFs, MYBC1 and WRKY44, more specifically regulate key branch points. This adds a layer of regulatory control that potentially balances anthocyanin and PA levels.
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