ArticleThe Plant cell2020
Establishment of Proximity-Dependent Biotinylation Approaches in Different Plant Model Systems.
Article in The Plant cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 75 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
75 citing papers in PubMed, 1 synthesis or guideline pooled it, 135 citations in OpenAlex.
- Meta-analysis of plant growth-promoting rhizobacteria interaction with host plants: implications for drought stress response gene expression.Frontiers in plant science · 2023Pooled it
- TurboID-based proteomic profiling reveals proxitome of the IRT1 metal transporter and new insight into IRT1 endocytosis.The New phytologist · 2026Article
- CDK8 phosphorylation of DELLA limits Mediator recruitment in gibberellin signaling.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Application of Proximity-Labeling Techniques in Plants: A Review of Successful Cases.International journal of molecular sciences · 2026Review
- Mapping the dynamic plant interactome: from in vitro assays to in vivo quantitative approaches.Plant methods · 2026Review
- CDK8 phosphorylation of DELLA limits Mediator recruitment in gibberellin signaling.bioRxiv : the preprint server for biology · 2026Article
- TOM1 family proteins: from cargo sorting to immune dysregulation and cancer.Cell communication and signaling : CCS · 2026Review
- Masters of perception: phosphorylation-dependent signaling in plants.The New phytologist · 2026Review
- Turbo-RIP: A Protocol for TurboID-based RNA Immunopurification to Map RNA Landscapes in Plant Biomolecular Condensates.Bio-protocol · 2026Article
- A CRISPR-based sequence proximity binding protein labelling system for scanning upstream regulatory proteins.Nature plants · 2026Article
- Illuminating Small Proteins Through HiBiT Blotting.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A combined biochemical and computational approach provides evidence for membrane remodelling by the structural scaffold of the endocytic TPLATE complex.Nature plants · 2025Article
- Modulating the activity of the APC/C regulator SISAMBA improves the sugar and antioxidant content of tomato fruits.Plant biotechnology journal · 2025Article
- Biotinylation Interferes with Protein Ubiquitylation and Turnover in Arabidopsis-A Cautionary Insight for Proximity Labeling in Ubiquitylation Proteome Studies.International journal of molecular sciences · 2025Article
- Split-YFP-coupled interaction-dependent TurboID identifies new functions of basal cell polarity inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Concerted transport and phosphorylation of diacylglycerol at ER-PM contact sites regulate phospholipid dynamics during stress.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Pupylation-based proximity labeling reveals regulatory factors in cellulose biosynthesis in Arabidopsis.Nature communications · 2025Article
- Identification of PsbS binding proteins inFrontiers in plant science · 2025Article
- dCas-Based Tools to Visualize Chromatin or Modify Epigenetic Marks at Specific Plant Genomic Loci.Methods in molecular biology (Clifton, N.J.) · 2025Review
- BioID in Bacteria: Selection of a Suitable Biotin Ligase for Proxeome Mapping.Methods in molecular biology (Clifton, N.J.) · 2025Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
17 authors at 5 institutions in 5 countries.
Funding
Abstract
Proximity labeling is a powerful approach for detecting protein-protein interactions. Most proximity labeling techniques use a promiscuous biotin ligase or a peroxidase fused to a protein of interest, enabling the covalent biotin labeling of proteins and subsequent capture and identification of interacting and neighboring proteins without the need for the protein complex to remain intact. To date, only a few studies have reported on the use of proximity labeling in plants. Here, we present the results of a systematic study applying a variety of biotin-based proximity labeling approaches in several plant systems using various conditions and bait proteins. We show that TurboID is the most promiscuous variant in several plant model systems and establish protocols that combine mass spectrometry-based analysis with harsh extraction and washing conditions. We demonstrate the applicability of TurboID in capturing membrane-associated protein interactomes using
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.