ArticleBMC veterinary research2020
A TaqMan-based real-time PCR assay for specific detection of novel duck reovirus in China.
Article in BMC veterinary research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Isolation, identification, and pathogenicity of Novel Duck Reovirus strain from ducklings in China.Poultry science · 2026Article
- TaqMan-probe-based multiplex real-time qPCR assay for simultaneous detection of Duck Tembusu virus, Novel duck reovirus, and Duck circovirus.Frontiers in microbiology · 2026Article
- Development of a TaqMan-Based Duplex Real-Time Quantitative RT-PCR for Detection and Differentiation of Muscovy Duck Reovirus and Novel Duck Reovirus.Pathogens (Basel, Switzerland) · 2025Article
- Development of duplex crystal digital PCR (dPCR) assay for detection and differentiation of NDRV and MDRV.Veterinary and animal science · 2025Article
- Update on Reovirus Infections in Waterfowls.Animals : an open access journal from MDPI · 2025Review
- Development of a TaqMan One-Step Quantitative PCR Assay for the Simultaneous Detection of Novel Goose Parvovirus and Novel Duck Reovirus.Microorganisms · 2025Article
- Development of an RT-qPCR Assay for the Detection of an Emerging Duck Egg-Reducing Syndrome.Veterinary sciences · 2025Article
- Metatranscriptomic analysis of the gut virome in Muscovy ducks reveals a novel duck reovirus potentially associated with hepatic and splenic hemorrhage.Frontiers in immunology · 2025Article
- Article
- Recombinant linear multiple epitopes of σB protein protect Muscovy ducks against novel duck reovirus infection.Frontiers in veterinary science · 2024Article
- Multiplex digital PCR: a superior technique to qPCR for the simultaneous detection of duck Tembusu virus, duck circovirus, and new duck reovirus.Frontiers in veterinary science · 2023Article
- Isolation and Pathogenicity Analysis of a Novel Orthoreovirus Caused the Outbreak of Duck Viral Arthritis in China.Transboundary and emerging diseases · 2023Article
- Identification and Pathogenicity Analysis of the Pathogen Causing Spotted Spleen in Muscovy Duck.Frontiers in veterinary science · 2022Article
- DDX1 from Cherry valley duck mediates signaling pathways and anti-NDRV activity.Veterinary research · 2021Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundIn China, Newly emerging duck reovirus (NDRV) variants have been causing major disease problems in cherry valley ducks. NDRV has the potential to cause high morbidity and 5-50% mortality rates. Severe hemorrhagic-necrosis in the liver and spleen were commonly seen in NDRV affected ducks. The availability of upgraded methods for rapid diagnosis of newly emerging DRV variants is crucial for successful DRV infection control and prevention.
resultsIn this study, we present a TaqMan-based real-time PCR assay (RT-qPCR) for the detection of NDRV infection. Using the conserved regions within the NDRV genome, we designed the specific primers and probe. The lower limit of detection for NDRV infection was 10 copies/μL (Ct values: 38.3) after the optimization of the RT-qPCR conditions. By cross-checking with other duck viral pathogens, no cross-reactivity was observed confirming the assay was highly specific for the detection of NDRV. Reproducibility of the RT-qPCR was confirmed by intra- and inter-assay variability was less than 2.91%(Intra-assay variability of Ct values: 0.07-1.48%; Interassay variability of Ct values: 0.49-2.91%). This RT-qPCR and conventional PCR (cPCR) detected one hundred and twenty samples of NDRV infection from different regions. The result shows that the positive rates were 94.17 and 84.17% respectively. The detection rate of RT-qPCR rapid detection assay was 10% higher than that of the cPCR method.
conclusionThis research developed a highly sensitive, specific, reproducible and versatile of RT-qPCR for quantitatively detecting NDRV. It can be used to study the pathogenesis and epidemiology investigation of NDRV.
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