ArticleBMC biotechnology2020
Vitrification for cryopreservation of 2D and 3D stem cells culture using high concentration of cryoprotective agents.
Article in BMC biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 31 citations in OpenAlex.
- Cryopreservation of human cortical organoids using vitrification.Frontiers in molecular neuroscience · 2026Article
- Article
- Current perspectives on the dynamic culture of mesenchymal stromal/stem cell spheroids.Stem cells translational medicine · 2025Review
- Characterization of the vitrification parameters for oviduct aggregates inFrontiers in veterinary science · 2025Article
- Testicular tissue cryopreservation and transplantation as a strategy for feline conservation: a review of research advances.Frontiers in veterinary science · 2025Review
- Cryopreservation of neuroectoderm on a pillar plate andbioRxiv : the preprint server for biology · 2024Article
- Specifics of Cryopreservation of Hydrogel Biopolymer Scaffolds with Encapsulated Mesenchymal Stem Cells.Polymers · 2024Article
- A tumour-spheroid manufacturing and cryopreservation process that yields a highly reproducible product ready for direct use in drug screening assays.Journal of the Royal Society, Interface · 2023Article
- Cryopreservation of tissues by slow-freezing using an emerging zwitterionic cryoprotectant.Scientific reports · 2023Article
- Review
- Development of a Vitrification Preservation Process for Bioengineered Epithelial Constructs.Cells · 2022Article
- In Situ Vitrification of Lung Cancer Organoids on a Microwell Array.Micromachines · 2021Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundVitrification is the most promising technology for successful cryopreservation of living organisms without ice crystal formation. However, high concentrations (up to ~ 6-8 M) of cryoprotective agents (CPAs) used in stem cell induce osmotic and metabolic injuries. Moreover, the application of conventional slow-freezing methods to cultures of 3-D organoids of stem cells in various studies, is limited by their size.
resultsIn this study, we evaluated the effect of high concentrations of CPAs including cytotoxicity and characterized human mesenchymal stem cell (MSC) at single cell level. The cell viability, cellular damage, and apoptotic mechanisms as well as the proliferation capacity and multipotency of cells subjected to vitrification were similar to those in the slow-freezing group. Furthermore, we identified the possibility of vitrification of size-controlled 3-D spheroids for cryopreservation of organoid with high survivability.
conclusionsOur results demonstrate successful vitrification of both single cell and spheroid using high concentration of CPAs in vitro without cytotoxicity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.