ArticleInternational journal of molecular sciences2020
Sperm Proteome after Interaction with Reproductive Fluids in Porcine: From the Ejaculation to the Fertilization Site.
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- Morphological and proteomic characterization of sperm in a beluga whale (Delphinapterus leucas):a case report.Veterinary research communications · 2026Article
- Sperm protein profiles and their correlation with DNA integrity and protamine deficiency in Donggala bullsVeterinary world · 2025Article
- Time to revise: impact of methodology on boar sperm capacitation in vitro via phosphotyrosine patterns.BMC veterinary research · 2025Review
- Dataset of Panda sperm proteome.Data in brief · 2024Article
- Relative Abundance of Spermadhesin-1 in the Seminal Plasma of Young Nellore Bulls Is in Agreement with Reproductive Parameters.Veterinary sciences · 2023Article
- The sperm-interacting proteome in the bovine isthmus and ampulla during the periovulatory period.Journal of animal science and biotechnology · 2023Article
- A proteomic approach to identifying spermatozoa proteins in Indonesian native Madura bulls.Frontiers in veterinary science · 2023Article
- The life history ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Female-induced selective modification of sperm protein SUMOylation-potential mechanistic insights into the non-random fertilization in humans.Journal of evolutionary biology · 2022Article
- Should All Fractions of the Boar Ejaculate Be Prepared for Insemination Rather Than Using the Sperm Rich Only?Biology · 2022Article
- Replacement of Albumin by Preovulatory Oviductal Fluid in Swim-Up Sperm Preparation Method Modifies Boar Sperm Parameters and Improves In Vitro Penetration of Oocytes.Animals : an open access journal from MDPI · 2021Article
- Molecular Determinants of Seminal Plasma on Sperm Biology and Fertility.International journal of molecular sciences · 2021Article
- Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Frontiers in cell and developmental biology · 2021Article
- A Comparative View on the Oviductal Environment during the Periconception Period.Biomolecules · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Ejaculated sperm are exposed to different environments before encountering the oocyte. However, how the sperm proteome changes during this transit remains unsolved. This study aimed to identify proteomic changes in boar sperm after incubation with male (seminal plasma, SP) and/or female (uterine fluid, UF; and oviductal fluid, OF) reproductive fluids. The following experimental groups were analyzed: (1) SP: sperm + 20% SP; 2) UF: sperm + 20% UF; 3) OF: sperm + 20% OF; 4) SP + UF: sperm + 20% SP + 20% UF; and (5) SP+OF: sperm + 20% SP + 20% OF. The proteome analysis, performed by HPLC-MS/MS, allowed the identification of 265 proteins. A total of 69 proteins were detected in the UF, SP, and SP + UF groups, and 102 proteins in the OF, SP, and SP + OF groups. Our results showed a higher number of proteins when sperm were incubated with only one fluid than when they were co-incubated with two fluids. Additionally, the number of sperm-interacting proteins from the UF group was lower than the OF group. In conclusion, the interaction of sperm with reproductive fluids alters its proteome. The description of sperm-interacting proteins in porcine species after co-incubation with male and/or female reproductive fluids may be useful to understand sperm transport, selection, capacitation, or fertilization phenomena.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.