Evidence map›Paper›PMID 32840789›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Investigation of Break-Induced Replication in Yeast.

Beth Osia, Rajula Elango, Juraj Kramara, Steven A Roberts, Anna Malkova

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.8field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Beth OsiaDepartment of Biology, University of Iowa, Iowa City, IA, USA.
Rajula ElangoDepartment of Medicine, Division of Hematology-Oncology, Cancer Research Institute, Harvard Medical School, Boston, MA, USA.
Juraj KramaraDepartment of Biology, University of Iowa, Iowa City, IA, USA.
Steven A RobertsSchool of Molecular Biosciences, Center for Reproductive Biology, Washington State University, Pullman, WA, USA.
Anna MalkovaDepartment of Biology, University of Iowa, Iowa City, IA, USA. anna-malkova@uiowa.edu.
University of Iowa · USBeth Israel Deaconess Medical Center · USWashington State University · US

Funding

Double strand break repair maelstrom: causes, mechanisms and genome destabilizing consequencesR35GM127006 · NIGMS · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI Anna L Malkova · 2018 to 2026
$3.8M
Mechanisms of genome instability induced by APOBEC Cytidine Deaminases & its impacts during cancer development - Diversity SupplementR01CA218112 · NCI · WASHINGTON STATE UNIVERSITY · PI ROBERTS, STEVEN A · 2017 to 2021
$2.2M
Identifying the link between the mode of telomere erosion and alternative telomere lengtheningR21ES030307 · NIEHS · UNIVERSITY OF IOWA · PI COMERON, JOSEP M, MALKOVA, ANNA L · 2019 to 2020
$408k
Suppressors and drivers of long-tract repair DNA synthesisR03ES029306 · NIEHS · UNIVERSITY OF IOWA · PI MALKOVA, ANNA L · 2018 to 2019
$153k
NCI NIH HHS R01 CA218112NIEHS NIH HHS R03 ES029306NIEHS NIH HHS R21 ES030307NIGMS NIH HHS R35 GM127006
6 · The paper itself

Abstract

Repair of double-strand DNA breaks (DSBs) is important for preserving genomic integrity and stability. Break-induced replication (BIR) is a mechanism aimed to repair one-ended double-strand DNA breaks, similar to those formed by replication fork collapse or by telomere erosion. Unlike S-phase replication, BIR is carried out by a migrating DNA bubble and is associated with conservative inheritance of newly synthesized DNA. This unusual DNA synthesis leads to high level of mutagenesis and chromosomal rearrangements during BIR. Here, we focus on several genetic and molecular methods to investigate BIR using our system in yeast Saccharomyces cerevisiae where BIR is initiated by a site-specific DNA break, and the repair involves two copies of chromosome III.

Indexed as

DNA Breaks, Double-StrandedChromosomes, FungalCytidine DeaminaseDNA ReplicationMutationRecombinational DNA RepairSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsCytidine DeaminaseSaccharomyces cerevisiae ProteinsAPOBECBreak-induced replicationContour-clamped homogenous electric field electrophoresisDouble-strand breakGross chromosomal rearrangementsHomologous recombinationSingle-stranded DNA

Identifiers

PMID32840789
PMCPMC9041317
OpenAlexW3081277989

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.