Evidence map›Paper›PMID 32840771›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Analysis of DNA Double-Strand Break End Resection and Single-Strand Annealing in S. pombe.

Zhenxin Yan, Sandeep Kumar, Grzegorz Ira

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Zhenxin YanDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
Sandeep KumarDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
Grzegorz IraDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA. gira@bcm.edu.
Baylor College of Medicine · US

Funding

Regulation of DNA recombination in Saccharomyces cerevisiaeR01GM080600 · NIGMS · BAYLOR COLLEGE OF MEDICINE · PI IRA, GRZEGORZ A · 2007 to 2024
$5.5M
Regulation of Initial Steps of Chromosomal Breaks RepairR01GM125650 · NIGMS · BAYLOR COLLEGE OF MEDICINE · PI IRA, GRZEGORZ A · 2018 to 2025
$2.9M
NIGMS NIH HHS R01 GM080600NIGMS NIH HHS R01 GM125650
6 · The paper itself

Abstract

DNA double-strand break (DSB) end resection is an essential step for homologous recombination. It generates 3' single-stranded DNA needed for the loading of the strand exchange proteins and DNA damage checkpoint proteins. To study the mechanism of end resection in fission yeast, we apply a robust, quantitative and inducible assay. Resection is followed at a single per genome DSB synchronously generated by the tet-inducible I-PpoI endonuclease. An additional assay to follow resection involves recombination between two direct repeats by single-strand annealing (SSA), since SSA requires extensive resection to expose two single-strand repeats for annealing. The kinetics of resection and SSA repair are then measured using Southern blots.

Indexed as

Recombinational DNA RepairBlotting, SouthernDNA Breaks, Double-StrandedDNA, FungalDNA, Single-StrandedEndodeoxyribonucleasesSchizosaccharomycesDNA, FungalDNA, Single-StrandedEndodeoxyribonucleasesI-Ppo endonucleaseDNA double-strand breakEnd resectionSingle-strand annealingSouthern blotting

Identifiers

PMID32840771
PMCPMC7647126
OpenAlexW3080163075

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.