Evidence map›Paper›PMID 32840225›Full record

ArticleInternational journal of stem cells2020

De Nan, Xiang Dou, Yankai Qi, Wenjun Zhang, Gaohong He, Xiujuan Zhang

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of stem cells, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

De NanState Key Laboratory of Fine Chemicals, School of Chemical Engineering, Panjin Campus, Dalian University of Technology, Panjin, China.
Xiang DouState Key Laboratory of Fine Chemicals, School of Chemical Engineering, Panjin Campus, Dalian University of Technology, Panjin, China.
Yankai QiState Key Laboratory of Fine Chemicals, School of Chemical Engineering, Panjin Campus, Dalian University of Technology, Panjin, China.
Wenjun ZhangState Key Laboratory of Fine Chemicals, School of Chemical Engineering, Panjin Campus, Dalian University of Technology, Panjin, China.
Gaohong HeState Key Laboratory of Fine Chemicals, School of Chemical Engineering, Panjin Campus, Dalian University of Technology, Panjin, China.
Xiujuan ZhangState Key Laboratory of Fine Chemicals, School of Chemical Engineering, Panjin Campus, Dalian University of Technology, Panjin, China.
Dalian University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Brain-derived neurotrophic factor (BDNF) exerts its survival-promoting effects on photoreceptors and retinal ganglion cells, however, delivery systems with little-to-no side effect are needed to sustain its controlled release and long-term efficacy. Our previous studies demonstrated that adipose-derived stem cells (ADSCs) are ideal delivery systems for gene therapy; moreover, ADSCs present unique properties like migration to damaged tissue sites, immunomodulation and anti-inflammation. Herein, we propose to employ ADSCs as the BDNF gene delivery vehicle. Different Analyses like flow cytometry, differentiation and cell proliferation assays etc demonstrated that BDNF were successfully transduced into ADSCs and the stemness of ADSCs was maintained even with the transduction. Real Time PCR and Western blot were used to measure mRNA and protein expressions of the BDNF-transduced ADSCs. The results demonstrated that the BDNF expression level of the lentiviral-BDNF transduced ADSCs is significantly increased and, moreover, enhanced the expression of other neurotrophic and downstream signaling factors. The data suggest that ADSCs are a reliable delivery vehicle for BDNF and could be used for the treatment of various diseases.

Indexed as

Adipose-derived mesenchymal stem cellsBrain-derived neurotrophic factorDrug deliveryLentiviral transductionNeural protection

Identifiers

PMID32840225
PMCPMC7691859
OpenAlexW3080399398

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.