ArticleNeurogastroenterology and motility2021
Characterization of putative interneurons in the myenteric plexus of human colon.
Article in Neurogastroenterology and motility, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Types of Neurons in the Myenteric Plexus of Human Gastric Corpus Identified by Multi-Layer Immunohistochemical Coding and Vagal Tracing.Neurogastroenterology and motility · 2026Article
- Tac1 deficiency reduces the severity of enteric bacterial infection.PLoS pathogens · 2026Article
- Subtypes of Enteric Neurons in Hirschsprung Disease: A Comparison of Fully Ganglionic and Transitional Zone.Neurogastroenterology and motility · 2026Article
- Molecular Diversity and Functional Implications of Mammalian Choline Acetyltransferases in Neuronal and Non-Neuronal Cells.International journal of molecular sciences · 2026Review
- A mathematical model of human oesophageal motility function.Royal Society open science · 2025Article
- From bugs to brain: unravelling the GABA signalling networks in the brain-gut-microbiome axis.Brain : a journal of neurology · 2025Review
- Recent advances in facilitating the translation of bioelectronic medicine therapies.Current opinion in biomedical engineering · 2025Article
- Extrinsic Innervation of Myenteric Plexus of Human Large Intestine Via Colonic Nerves.Cellular and molecular gastroenterology and hepatology · 2025Article
- Over 30 Years of DiI Use for Human Neuroanatomical Tract Tracing: A Scoping Review.Biomolecules · 2024Article
- The parasympathetic and sensory innervation of the proximal and distal colon in male mice.Frontiers in neuroanatomy · 2024Article
- Distribution, quantification, and characterization of substance P enteric neurons in the submucosal and myenteric plexuses of the porcine colon.Cell and tissue research · 2024Article
- Serotonergic Control of Gastrointestinal Development, Motility, and Inflammation.Comprehensive Physiology · 2023Article
- The enteric nervous system.Physiological reviews · 2023Review
- Characterization of viscerofugal neurons in human colon by retrograde tracing and multi-layer immunohistochemistry.Frontiers in neuroscience · 2023Article
- New insight on the enteric cholinergic innervation of the pig colon by central and peripheral nervous systems: reduction by repeated loperamide administration.Frontiers in neuroscience · 2023Article
- Sympathetic Pathways Target Cholinergic Neurons in the Human Colonic Myenteric Plexus.Frontiers in neuroscience · 2022Article
- Classification of human enteric neurons.Histochemistry and cell biology · 2021Review
- The Baseline Structure of the Enteric Nervous System and Its Role in Parkinson's Disease.Life (Basel, Switzerland) · 2021Review
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Authors and funding
8 authors.
Funding
Abstract
backgroundThe enteric nervous system contains multiple classes of neurons, distinguishable by morphology, immunohistochemical markers, and projections; however, specific combinations differ between species. Here, types of enteric neurons in human colon were characterized immunohistochemically, using retrograde tracing combined with multiple labeling immunohistochemistry, focussing on non-motor neurons.
methodsThe fluorescent carbocyanine tracer, DiI, was applied to the myenteric plexus in ex vivo preparations, filling neurons projecting within the plexus. Limits of projection lengths of motor neurons were established, allowing them to be excluded from the analysis. Long ascending and descending interneurons were then distinguished by labeling for discriminating immunohistochemical markers: calbindin, calretinin, enkephalin, 5-hydroxytryptamine, nitric oxide synthase, and substance P. These results were combined with a previous published study in which nitric oxide synthase and choline acetyltransferase immunoreactivities were established. KEY
resultsLong ascending neurons (with projections longer than 8 mm, which excludes more than 95% motor neurons) formed four types, in descending order of abundance, defined by immunoreactivity for: (a) ChAT+/ENK+, (b) ChAT+/ENK+/SP+, (c) ChAT+/Calb+, and (d) ChAT+/ENK+/Calb+. Long descending neurons, up to 70 mm long also formed at least four types, distinguished by immunoreactivity for (a) NOS + cells (without ChAT), (b) ChAT+/NOS+, (c) ChAT+/Calret+, and (d) ChAT+/5HT + cells (with or without NOS). CONCLUSIONS AND INFERENCES: Long interneurons, which do not innervate muscularis externa, are likely to coordinate neural activity over distances of many centimeters along the colon. Characterizing their neurochemical coding provides a basis for understanding their roles, investigating their connectivity, and building a comprehensive account of human colonic enteric neurons.
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