Evidence map›Paper›PMID 32839935›Full record

ArticleMolecular and cellular biochemistry2020

Circ_0079593 facilitates proliferation, metastasis, glucose metabolism and inhibits apoptosis in melanoma by regulating the miR-516b/GRM3 axis.

Jiajing Lu, Ying Li

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular and cellular biochemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 1 pooled it
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.

  1. Circular RNA as a Potential Biomarker for Melanoma: A Systematic Review.Frontiers in cell and developmental biology · 2021
    Pooled it
  2. Review
  3. Review
  4. Omics in Cutaneous Melanoma.Asian Pacific journal of cancer prevention : APJCP · 2025
    Review
  5. Cancer and neurotransmitter receptors.Chinese medical journal · 2025
    Review
  6. Review
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  8. Article
  9. Article
  10. Review
  11. Circ_0081054 facilitates melanoma development via sponging miR-637 and regulating RAB9A.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2023
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Jiajing LuDepartment of Dermatology, Shanghai Skin Disease Hospital, No. 1278 Baode Road, Jing'an District, Shanghai, 200443, China. kct5spu@163.com.ORCID http://orcid.org/0000-0002-2456-8958
Ying LiDepartment of Dermatology, Shanghai Skin Disease Hospital, No. 1278 Baode Road, Jing'an District, Shanghai, 200443, China.
Shanghai Skin Disease Hospital · CN

Funding

Shanghai Municipal Commission of Health and Family Planning 20174Y0029
6 · The paper itself

Abstract

Many studies confirm that circular RNA (circRNA) plays an important regulatory role in the malignant progression of cancer, including melanoma. However, the role of a novel circRNA, circ_0079593, in melanoma is unclear. The expression levels of circ_0079593 and miR-516b were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation was measured by cell counting kit-8 (CCK-8) assay, and cell migration and invasion were evaluated using transwell assay. Meanwhile, western blot (WB) analysis was employed to determine the levels of proliferation and metastasis-related proteins, as well as metabotropic glutamate receptor 3 (GRM3) protein. Furthermore, cell apoptosis was tested by detecting the cell apoptosis rate and Caspase-3 activity. The glucose consumption and lactate production of cells were measured to evaluate cell glucose metabolism. Moreover, dual-luciferase reporter assay and biotin-labeled RNA pull-down assay were used to confirm the interaction between miR-516b and circ_0079593 or GRM3. In addition, mice xenograft models were constructed to explore the effect of circ_0079593 on melanoma tumor growth in vivo. Our results discovered that circ_0079593 was highly expressed in melanoma, and its silencing suppressed melanoma cell proliferation, migration, invasion, glucose metabolism and promoted apoptosis. Moreover, we found that circ_0079593 could serve as a sponge of miR-516b, and miR-516b could target GRM3 in melanoma. The rescue experiments revealed that both miR-516b inhibitor and GRM3 overexpression could reverse the inhibition effect of circ_0079593 knockdown on melanoma progression. Additionally, in vivo experiments also revealed that circ_0079593 interference suppressed melanoma tumor growth. Our study concluded that circ_0079593 accelerated melanoma progression via upregulating GRM3 by sponging miR-516b, which suggested that circ_0079593 had the potential to be a new therapeutic biomarker for melanoma.

Indexed as

AnimalsApoptosisCell Line, TumorCell MovementCell ProliferationGlucoseHumansMaleMelanomaMiceMice, NudeMicroRNAsNeoplasm MetastasisReceptors, AMPARNA, CircularXenograft Model Antitumor AssaysGlucoseglutamate receptor ionotropic, AMPA 3MicroRNAsMIRN516 microRNA, humanReceptors, AMPARNA, Circularcirc_0079593GRM3MelanomamiR-516b

Identifiers

PMID32839935
OpenAlexW3080464487

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.