Evidence map›Paper›PMID 32839773›Full record

ArticlebioRxiv : the preprint server for biology2021

A Human-Immune-System (HIS) humanized mouse model (DRAGA: HLA-A2. HLA-DR4. Rag1 KO.IL-2Rγc KO. NOD) for COVID-19.

Teodor-D Brumeanu, Pooja Vir, Ahmad Faisal Karim, Swagata Kar, Dalia Benetiene, Megan Lok, Jack Greenhouse, Tammy Putmon-Taylor, Christopher Kitajewski, Kevin K Chung and 2 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 6 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Teodor-D BrumeanuUniformed Services University of the Health Sciences, Department of Medicine, Division of Immunology, Bethesda, MD 20814, U.S.A.
Pooja VirUniformed Services University of the Health Sciences, Department of Medicine, Division of Immunology, Bethesda, MD 20814, U.S.A.
Ahmad Faisal KarimUniformed Services University of the Health Sciences, Department of Medicine, Division of Immunology, Bethesda, MD 20814, U.S.A.
Swagata KarBioqual Inc., Rockville, MD 20852, U.S.A.
Dalia BenetieneBioqual Inc., Rockville, MD 20852, U.S.A.
Megan LokBioqual Inc., Rockville, MD 20852, U.S.A.
Jack GreenhouseBioqual Inc., Rockville, MD 20852, U.S.A.
Tammy Putmon-TaylorBioqual Inc., Rockville, MD 20852, U.S.A.
Christopher KitajewskiBioqual Inc., Rockville, MD 20852, U.S.A.
Kevin K ChungUniformed Services University of the Health Sciences, Department of Medicine, Division of Immunology, Bethesda, MD 20814, U.S.A.
Kathleen P PrattUniformed Services University of the Health Sciences, Department of Medicine, Division of Immunology, Bethesda, MD 20814, U.S.A.
Sofia A CasaresUniformed Services University of the Health Sciences, Department of Medicine, Division of Immunology, Bethesda, MD 20814, U.S.A.
Bioqual · USUniformed Services University of the Health Sciences · USNaval Medical Research Command · US

Funding

Design of Less Immunogenic Factor VIII ProteinsR01HL130448 · NHLBI · HENRY M. JACKSON FDN FOR THE ADV MIL/MED · PI PRATT, KATHLEEN PALMER · 2016 to 2019
$1.5M
NHLBI NIH HHS R01 HL130448
6 · The paper itself

Abstract

We report the first Human Immune System (HIS)-humanized mouse model ("DRAGA": HLA-A2.HLA-DR4.Rag1KO.IL-2RγcKO.NOD) for COVID-19 research. This mouse is reconstituted with human cord blood-derived, HLA-matched hematopoietic stem cells. It engrafts human epi/endothelial cells expressing the human ACE2 receptor for SARS-CoV-2 and TMPRSS2 serine protease co-localized on lung epithelia. HIS-DRAGA mice sustained SARS-CoV-2 infection, showing deteriorated clinical condition, replicating virus in the lungs, and human-like lung immunopathology including T-cell infiltrates, microthrombi and pulmonary sequelae. Among T-cell infiltrates, lung-resident (CD103

Indexed as

COVID-19HIS-humanized mouse modellung immunopathologySARS-CoV-2

Identifiers

PMID32839773
PMCPMC7444284
OpenAlexW3066993807

What OpenQuestion holds

Textmetadata
LicenceCC0
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.