ArticleNature chemical biology2020
A bio-inspired cell-free system for cannabinoid production from inexpensive inputs.
Article in Nature chemical biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 56 citations in OpenAlex.
- Reconstructing the hydrogenobyrinic acid synthetic toolkit by combining cell-free systems and metabolic engineering.Synthetic and systems biotechnology · 2026Article
- Cell-Free Synthesis of Cannabistilbene I: A Dual Acting Anti-Inflammatory fromJournal of natural products · 2026Article
- Propellane Alkaloid Biosynthesis and Total Synthesis via Interrupted Reaction Pathways.ACS central science · 2026Article
- Glycosylated cannabinoids inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Microbial biosynthesis of rare cannabinoids.Journal of industrial microbiology & biotechnology · 2024Article
- The utility of Streptococcus mutans undecaprenol kinase for the chemoenzymatic synthesis of diverse non-natural isoprenoids.Bioorganic chemistry · 2024Article
- Rational Design and Modification of NphB for Cannabinoids Biosynthesis.Molecules (Basel, Switzerland) · 2024Article
- Discovery of a terpene synthase synthesizing a nearly non-flexible eunicellane reveals the basis of flexibility.Nature communications · 2024Article
- Review
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- ATP-free in vitro biotransformation of starch-derived maltodextrin into poly-3-hydroxybutyrate via acetyl-CoA.Nature communications · 2024Article
- Iterative design of training data to control intricate enzymatic reaction networks.Nature communications · 2024Article
- Article
- EnzymeMap: curation, validation and data-driven prediction of enzymatic reactions.Chemical science · 2023Article
- Article
- A synthetic cell-free 36-enzyme reaction system for vitamin BNature communications · 2023Article
- Biotechnological Fungal Platforms for the Production of Biosynthetic Cannabinoids.Journal of fungi (Basel, Switzerland) · 2023Review
- Biosynthesis of cannabigerol and cannabigerolic acid: the gateways to further cannabinoid production.Synthetic biology (Oxford, England) · 2023Article
- Merging enzymatic and synthetic chemistry with computational synthesis planning.Nature communications · 2022Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Moving cannabinoid production away from the vagaries of plant extraction and into engineered microbes could provide a consistent, purer, cheaper and environmentally benign source of these important therapeutic molecules, but microbial production faces notable challenges. An alternative to microbes and plants is to remove the complexity of cellular systems by employing enzymatic biosynthesis. Here we design and implement a new cell-free system for cannabinoid production with the following features: (1) only low-cost inputs are needed; (2) only 12 enzymes are employed; (3) the system does not require oxygen and (4) we use a nonnatural enzyme system to reduce ATP requirements that is generally applicable to malonyl-CoA-dependent pathways such as polyketide biosynthesis. The system produces ~0.5 g l
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.