ArticleCells2020
Modulation of Human Adipose Stem Cells' Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro.
Article in Cells, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 13 citations in OpenAlex.
- Research trends and hotspots in Adipose-Derived Stem Cell applications for peripheral nerve regeneration from 2000 to 2025: a bibliometrics visualization study.Brain & spine · 2026Review
- Beyond conventional therapies: MSCs in the battle against nerve injury.Regenerative therapy · 2025Review
- Advances in 3D printing combined with tissue engineering for nerve regeneration and repair.Journal of nanobiotechnology · 2025Review
- Adipose stem cells in tissue regeneration and repair: From bench to bedside.Regenerative therapy · 2023Review
- Heparin-Based Hydrogel Micropatches with Human Adipose-Derived Stem Cells: A Promising Therapeutic Approach for Neuropathic Pain Relief.Biomedicines · 2023Article
- Mesenchymal Stem Cells in Nerve Tissue Engineering: Bridging Nerve Gap Injuries in Large Animals.International journal of molecular sciences · 2023Review
- Improving Schwann Cell Differentiation from Human Adipose Stem Cells with Metabolic Glycoengineering.Cells · 2023Article
- Synergy of human platelet lysate and laminin to enhance the neurotrophic effect of human adipose-derived stem cells.Neural regeneration research · 2022Article
- Optimized Decellularization Protocol for Large Peripheral Nerve Segments: Towards Personalized Nerve Bioengineering.Bioengineering (Basel, Switzerland) · 2022Article
- Adipose tissue in bone regeneration - stem cell source and beyond.World journal of stem cells · 2022Review
- Neurogenic and Neuroprotective Potential of Stem/Stromal Cells Derived from Adipose Tissue.Cells · 2021Article
- Antibacterial agent-releasing scaffolds in dental tissue engineering.Journal of advanced periodontology & implant dentistry · 2021Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report on a potential strategy involving the exogenous neurotrophic factors (NTF) for enhancing the neurotrophic capacity of human adipose stem cells (ASC) in vitro. For this, ASC were stimulated for three days using NTF, i.e., nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT3), NT4, glial cell-derived neurotrophic factor (GDNF), and ciliary neurotrophic factor (CNTF). The resulting conditioned medium (CM) as well as individual NTF exhibited distinct effects on axonal outgrowth from dorsal root ganglion (DRG) explants. In particular, CM derived from NT3-stimulated ASC (CM-NT3-ASC) promoted robust axonal outgrowth. Subsequent transcriptional analysis of DRG cultures in response to CM-NT3-ASC displayed significant upregulation of STAT-3 and GAP-43. In addition, phosphoproteomic analysis of NT3-stimulated ASC revealed significant changes in the phosphorylation state of different proteins that are involved in cytokine release, growth factors signaling, stem cell maintenance, and differentiation. Furthermore, DRG cultures treated with CM-NT3-ASC exhibited significant changes in the phosphorylation levels of proteins involved in tubulin and actin cytoskeletal pathways, which are crucial for axonal growth and elongation. Thus, the results obtained at the transcriptional, proteomic, and cellular level reveal significant changes in the neurotrophic capacity of ASC following NT3 stimulation and provide new options for improving the axonal growth-promoting potential of ASC in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.