Evidence map›Paper›PMID 32838950›Full record

ArticleVirology2020

Cytomegalovirus infection elicits a conserved chemokine response from human and guinea pig amnion cells.

Dira S Putri, Zachary W Berkebile, Hiba J Mustafa, Claudia Fernández-Alarcón, Juan E Abrahante, Mark R Schleiss, Craig J Bierle

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.3field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Maternal Immune Activation: Implications for Congenital Heart Defects.Clinical reviews in allergy & immunology · 2025
    Review
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Dira S PutriDepartment of Pediatrics, Division of Pediatric Infectious Diseases and Immunology, University of Minnesota, Minneapolis, MN, USA. Electronic address: dputri@umn.edu.
Zachary W BerkebileDepartment of Pediatrics, Division of Pediatric Infectious Diseases and Immunology, University of Minnesota, Minneapolis, MN, USA. Electronic address: berk0222@umn.edu.
Hiba J MustafaDepartment of Obstetrics, Gynecology and Women's Health, Division of Maternal-Fetal Medicine, University of Minnesota, Minneapolis, MN, USA. Electronic address: musta062@umn.edu.
Claudia Fernández-AlarcónDepartment of Pediatrics, Division of Pediatric Infectious Diseases and Immunology, University of Minnesota, Minneapolis, MN, USA. Electronic address: ferna128@umn.edu.
Juan E AbrahanteInformatics Institute, University of Minnesota, Minneapolis, MN, USA. Electronic address: abrah023@umn.edu.
Mark R SchleissDepartment of Pediatrics, Division of Pediatric Infectious Diseases and Immunology, University of Minnesota, Minneapolis, MN, USA. Electronic address: schleiss@umn.edu.
Craig J BierleDepartment of Pediatrics, Division of Pediatric Infectious Diseases and Immunology, University of Minnesota, Minneapolis, MN, USA. Electronic address: cjbierle@umn.edu.
University of Minnesota · US

Funding

University of Minnesota Clinical and Translational Science Institute (UMN CTSI)UL1TR002494 · NCATS · UNIVERSITY OF MINNESOTA · PI BLAZAR, BRUCE R, WEISDORF, DANIEL J · 2018 to 2022
$34.9M
Optimized Vaccines Against Congenital Infection and Maternal Reinfection with CMVR01HD079918 · NICHD · UNIVERSITY OF MINNESOTA · PI SCHLEISS, MARK R. · 2015 to 2019
$1.6M
Defining the viral determinants of cytomegalovirus transplacental transmissionR21HD087496 · NICHD · UNIVERSITY OF MINNESOTA · PI BIERLE, CRAIG JOHN · 2017 to 2018
$420k
NCATS NIH HHS UL1 TR002494NICHD NIH HHS R01 HD079918NICHD NIH HHS R21 HD087496
6 · The paper itself

Abstract

Human cytomegalovirus (HCMV) infects the chorioamnion, but whether these infections cause fetal membrane dysfunction remains poorly understood. We sought to assess whether guinea pig cytomegalovirus (GPCMV) infects amnion-derived cells in vitro, compare the inflammatory response of amnion cells to GPCMV and HCMV, and determine if GPCMV infects the amnion in vivo. We found that GPCMV replicates in primary guinea pig amnion derived cells and HPV16 E6/E7-transduced amniotic epithelial cells (AEC[E6/E7]s). HCMV and GPCMV infection of amnion cells increased the transcription of the chemokines CCL5/Ccl5, CXCL8/Cxcl8, and CXCL10/Cxcl10. Myd88-knockdown decreased Ccl5 and Cxc8 transcription in GPCMV-infected AEC[E6/E7]s. GPCMV was detected in the guinea pig amnion after primary maternal infection, revealing that guinea pigs are an appropriate model to study fetal membrane physiology after cytomegalovirus infection. As inflammation is known to cause fetal membrane weakening, the amnion's response to cytomegalovirus infection may cause preterm birth and other adverse pregnancy outcomes.

Indexed as

AmnionAnimalsChemokine CCL5Chemokine CXCL10ChemokinesCytomegalovirusCytomegalovirus InfectionsFemaleGuinea PigsHumansInterleukin-8PregnancyPregnancy ComplicationsChemokine CCL5Chemokine CXCL10ChemokinesInterleukin-8Amnion cellsCytomegalovirusFetal membranesGuinea pigInflammation

Identifiers

PMID32838950
PMCPMC7447834
OpenAlexW3036423839

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.