ArticleWorld journal of surgical oncology2020
Investigation of the SPAG5 gene expression and amplification related to the NuMA mRNA levels in breast ductal carcinoma.
Article in World journal of surgical oncology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed, 10 citations in OpenAlex.
- mtDNA copy number/BioImpacts : BI · 2025Article
- K-Ras4A Plays a More Significant Role than K-Ras4B in Ductal Carcinoma of Breast.Iranian biomedical journal · 2023Article
- Down-regulation ofInternational journal of molecular epidemiology and genetics · 2023Article
- Comprehensive analysis of the cancer driver genes in breast cancer demonstrates their roles in cancer prognosis and tumor microenvironment.World journal of surgical oncology · 2021Article
- In silico analysis of differentially expressed genesets in metastatic breast cancer identifies potential prognostic biomarkers.World journal of surgical oncology · 2021Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
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Abstract
backgroundThe cell proliferative markers are very important in breast cancer. Since SPAG5 and NuMA proteins play a significant role in the mitosis regulatory network and cell division, we aimed to study their mRNA levels as well as SPAG5 gene amplification correlated to clinicopathological status in ductal carcinoma of the breast.
methodsSPAG5 and NuMA gene expressions were investigated in 40 breast cancer tissues and normal adjacent tissues via real-time PCR. PUM1 was selected as the reference gene. QMF PCR method was applied to study SPAG5 gene amplification and AGBL2, BOD1L, and POR were designated as internal control genes. Gene amplification was determined by calculating a dosage quotient for each DNA fragment.
resultsIncreased SPAG5 mRNA expression was detected in breast cancer tissues (p = 0.005) and related to tumor size. No significant difference was observed between NuMA gene expression level in tumor tissue and the normal adjacent tissue (p = 0.56). However, we observed that NuMA expression was significantly increased in ER-positive tumor tissues. There was no clear correlation pattern between SPAG5 and NuMA mRNA levels (r = 0.33). Seventeen percent of tissues showed complete amplification in SPAG5 gene fragments.
conclusionOur results were consistent with the previous publications regarding SPAG5 gene expression and amplification in breast cancer with an emphasis on the prominent role of this protein in tumor pathogenesis. Our results failed to yield any correlation between SPAG5 and NuMA mRNA levels which implies independence of these genes in breast cancer pathogenesis.
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