Evidence map›Paper›PMID 32835602›Full record

ArticlemAbs

Novel monoclonal antibodies to the SERINC5 HIV-1 restriction factor detect endogenous andvirion-associated SERINC5.

Sebastian Molnar, Lindsay Wieczorek, Michelle Zemil, Bianca Schulte, Elizabeth Martinez, Syna Gift, Lan Tang, Hendrik Streeck, Robert A Gramzinski, Nelson L Michael and 2 more

Open access · goldAbstract readVideo-Audio Media
PubMed Full text at PMC Publisher
In one paragraph

Article in mAbs. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 2 countries.

Sebastian MolnarMilitary HIV Research Program, Walter Reed Army Institute of Research , Silver Spring, MD, USA.
Lindsay WieczorekMilitary HIV Research Program, Walter Reed Army Institute of Research , Silver Spring, MD, USA.
Michelle ZemilMilitary HIV Research Program, Walter Reed Army Institute of Research , Silver Spring, MD, USA.
Bianca SchulteInstitute for Virology, University Hospital Bonn , Bonn, Germany.
Elizabeth MartinezMilitary HIV Research Program, Walter Reed Army Institute of Research , Silver Spring, MD, USA.
Syna GiftMilitary HIV Research Program, Walter Reed Army Institute of Research , Silver Spring, MD, USA.
Lan TangBiologics Department, GenScript , Piscataway, NJ, USA.
Hendrik StreeckInstitute for Virology, University Hospital Bonn , Bonn, Germany.
Robert A GramzinskiMilitary HIV Research Program, Walter Reed Army Institute of Research , Silver Spring, MD, USA.
Nelson L MichaelMilitary HIV Research Program, Walter Reed Army Institute of Research , Silver Spring, MD, USA.
Gordon JoyceMilitary HIV Research Program, Walter Reed Army Institute of Research , Silver Spring, MD, USA.
Victoria R PolonisMilitary HIV Research Program, Walter Reed Army Institute of Research , Silver Spring, MD, USA.
Walter Reed Army Institute of Research · USHenry M. Jackson Foundation · USUniversity Hospital Bonn · DEAllscripts (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

SERINC5 is a multi-pass transmembrane protein that is thought to play a role in serine incorporation during cellular membrane biosynthesis. This protein has also been identified as a human immunodeficiency virus Type 1 (HIV-1) restriction factor. The paucity of monoclonal antibodies (mAbs) against SERINC5 has posed a challenge for the study of the endogenous protein. Here we report the development of novel anti-SERINC5 mAbs that target three distinct loops on the protein. We demonstrate that these SERINC5 mAbs can be used to detect endogenously expressed SERINC5 protein in various cell lines using Western blot, whole-cell ELISA, flow cytometry, and immunocytochemistry. We further show that some of these antibodies can detect SERINC5 that is present in HIV-1 viral stocks. These antibodies will aid in the characterization of the functions and mechanisms of action of SERINC5 in different cell types.

Indexed as

AnimalsAntibodies, Monoclonal, Murine-DerivedHEK293 CellsHIV-1HumansMembrane ProteinsMiceMice, Inbred BALB CVirionAntibodies, Monoclonal, Murine-DerivedMembrane ProteinsSERINC5 protein, humanHIV-1 restriction factorimmunocytochemistrymonoclonal antibodymulti-pass transmembrane proteinSERINC5serine incorporatorvirus capture

Identifiers

PMID32835602
PMCPMC7531522
OpenAlexW3081233970

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.