ArticleAngewandte Chemie (International ed. in English)2020
An Integrated Multi-Function Heterogeneous Biochemical Circuit for High-Resolution Electrochemistry-Based Genetic Analysis.
Article in Angewandte Chemie (International ed. in English), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 53 citations in OpenAlex.
- Multiple TMA-aided CRISPR/Cas13a platform for highly sensitive detection of IL-15 to predict immunotherapeutic response in nasopharyngeal carcinoma.Journal for immunotherapy of cancer · 2023Article
- Application of CRISPR Cas Systems for Biosensing.Biosensors · 2023Article
- The Trend of CRISPR-Based Technologies in COVID-19 Disease: Beyond Genome Editing.Molecular biotechnology · 2023Review
- Clustered Regularly Interspaced short palindromic repeats-Based Microfluidic System in Infectious Diseases Diagnosis: Current Status, Challenges, and Perspectives.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- A PAM-free CRISPR/Cas12a ultra-specific activation mode based on toehold-mediated strand displacement and branch migration.Nucleic acids research · 2022Article
- Sensor technologies for quality control in engineered tissue manufacturing.Biofabrication · 2022Review
- Innovations and Challenges in Electroanalytical Tools for Rapid Biosurveillance of SARS-CoV-2.Advanced materials technologies · 2022Article
- Unified-amplifier based primer exchange reaction (UniAmPER) enabled detection of SARS-CoV-2 from clinical samples.Sensors and actuators. B, Chemical · 2022Article
- Rapid and High-Throughput SARS-CoV-2 RNA Detection without RNA Extraction and Amplification by Using a Microfluidic Biochip.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Research progress of CRISPR-based biosensors and bioassays for molecular diagnosis.Frontiers in bioengineering and biotechnology · 2022Review
- CRISPR-Cas based virus detection: Recent advances and perspectives.Biosensors & bioelectronics · 2021Review
- CRISPR-based tools: Alternative methods for the diagnosis of COVID-19.Clinical biochemistry · 2021Review
- Chemical Translational Biology-Guided Molecular Diagnostics: The Front Line To Mediate the Current SARS-CoV-2 Pandemic.Chembiochem : a European journal of chemical biology · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Modular construction of an autonomous and programmable multi-functional heterogeneous biochemical circuit that can identify, transform, translate, and amplify biological signals into physicochemical signals based on logic design principles can be a powerful means for the development of a variety of biotechnologies. To explore the conceptual validity, we design a CRISPR-array-mediated primer-exchange-reaction-based biochemical circuit cascade, which probes a specific biomolecular input, transform the input into a structurally accessible form for circuit wiring, translate the input information into an arbitrary sequence, and finally amplify the prescribed sequence through autonomous formation of a signaling concatemer. This upstream biochemical circuit is further wired with a downstream electrochemical interface, delivering an integrated bioanalytical platform. We program this platform to directly analyze the genome of SARS-CoV-2 in human cell lysate, demonstrating the capability and the utility of this unique integrated system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.