ArticleThe Journal of antimicrobial chemotherapy2020
Novel IncFII plasmid harbouring blaNDM-4 in a carbapenem-resistant Escherichia coli of pig origin, Italy.
Article in The Journal of antimicrobial chemotherapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 31 citations in OpenAlex.
- Study on drug resistance, biofilm formation, phylogenetic and virulence gene analysis of Escherichia coli from diarrheic lambs.BMC veterinary research · 2026Article
- Genomics of NDM-5 producingJAC-antimicrobial resistance · 2025Article
- Characterization of antibiotic determinants and heavy metal resistance genes inMicrobial genomics · 2025Article
- Genomic epidemiology of antimicrobial resistance determinants in Chinese swine farmFrontiers in microbiology · 2025Article
- Characterization of Antimicrobial Resistance inAnimals : an open access journal from MDPI · 2024Article
- The European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2021-2022.EFSA journal. European Food Safety Authority · 2024Article
- Reservoirs of antimicrobial resistance in the context of One Health.Current opinion in microbiology · 2023Review
- Genomics Insight intoAntibiotics (Basel, Switzerland) · 2023Article
- The European Union Summary Report on Antimicrobial Resistance in zoonotic and indicator bacteria from humans, animals and food in 2020/2021.EFSA journal. European Food Safety Authority · 2023Article
- Evidence of structural rearrangements in ESBL-positive pESI(like) megaplasmids of S.Infantis.FEMS microbiology letters · 2023Article
- Evaluation and validation of laboratory procedures for the surveillance of ESBL-, AmpC-, and carbapenemase-producingFrontiers in microbiology · 2023Article
- Comparison of Two Distinct Subpopulations of Klebsiella pneumoniae ST16 Co-Occurring in a Single Patient.Microbiology spectrum · 2022Article
- Survey on Carbapenem-Resistant Bacteria in Pigs at Slaughter and Comparison with Human Clinical Isolates in Italy.Antibiotics (Basel, Switzerland) · 2022Article
- The hazard of carbapenemase (OXA-181)-producingFrontiers in microbiology · 2022Article
- Comparing Long-Read Assemblers to Explore the Potential of a Sustainable Low-Cost, Low-Infrastructure Approach to Sequence Antimicrobial Resistant Bacteria With Oxford Nanopore Sequencing.Frontiers in microbiology · 2022Article
- Editorial: Antimicrobial Resistance as a Global Public Health Problem: How Can We Address It?Frontiers in public health · 2020Article
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11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesTo the best of our knowledge, we describe the first evidence in Europe of an MDR, blaNDM-4-positive Escherichia coli isolated from a food-producing animal, harboured by a novel IncFII plasmid of which we report the complete sequence.
methodsOne blaNDM-4-positive E. coli isolated in 2019 from the caecal contents of a fattening pig in Italy was in-depth characterized by combined bioinformatic analysis of Oxford Nanopore long reads and Illumina short reads, for in silico typing, determination of the blaNDM-4 genetic context and full reconstruction of the blaNDM-4-carrying plasmid.
resultsThe isolate belonged to ST641 and to the genoserotype O108:H23 and tested positive for different virulence genes and plasmid replicons. The MDR phenotype of resistance to all β-lactams, carbapenems, sulfamethoxazole and trimethoprim was mediated by blaTEM-1B, blaNDM-4, sul1/sul3 and dfrA12, respectively. The blaNDM-4 gene was harboured by a novel 53 043 bp IncFII plasmid (pMOL412_FII) composed of four main genetic regions, including an MDR region (MRR-NDM-4) of 16 kb carrying blaNDM-4 and several antimicrobial resistance genes located in a class 1 integron. pMOL412_FII was closely related to another ∼90.3 kb plasmid (pM109_FII) harbouring blaNDM-4 in an E. coli isolated from a human patient in Myanmar.
conclusionsTo the best of our knowledge, we have identified for the first time in Europe an NDM-producing Enterobacterales in livestock and resolved the complete sequence of the novel pMOL412_FII plasmid harbouring blaNDM-4 in an MRR. A global One Health approach, comparing genomic data from different sources and geographical areas, may help to trace back and control possible plasmid-borne carbapenemase gene transmission between animals and humans and along the food chain at international level.
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