Evidence map›Paper›PMID 32833532›Full record

ArticleThe CRISPR journal2020

Targeted RNA Knockdown by a Type III CRISPR-Cas Complex in Zebrafish.

Thomas Fricke, Dalia Smalakyte, Maciej Lapinski, Abhishek Pateria, Charles Weige, Michal Pastor, Agnieszka Kolano, Cecilia Winata, Virginijus Siksnys, Gintautas Tamulaitis and 1 more

Open access · hybridAbstract read
In one paragraph

Article in The CRISPR journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 33 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Insight into crRNA Processing inInternational journal of molecular sciences · 2025
    Article
  6. Article
  7. Enhanced RNA-targeting CRISPR-Cas technology in zebrafish.bioRxiv : the preprint server for biology · 2024
    Article
  8. Review
  9. CRISPR technologies for genome, epigenome and transcriptome editing.Nature reviews. Molecular cell biology · 2024
    Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. CRISPR-based engineering of RNA viruses.bioRxiv : the preprint server for biology · 2023
    Article
  15. Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Thomas FrickeInternational Institute of Molecular and Cell Biology, Warsaw, Poland; Institute of Biochemistry and Biophysics, Warsaw, Poland.
Dalia SmalakyteInstitute of Biotechnology, Vilnius University, Vilnius, Lithuania; Institute of Biochemistry and Biophysics, Warsaw, Poland.
Maciej LapinskiInternational Institute of Molecular and Cell Biology, Warsaw, Poland; Institute of Biochemistry and Biophysics, Warsaw, Poland.
Abhishek PateriaInternational Institute of Molecular and Cell Biology, Warsaw, Poland; Institute of Biochemistry and Biophysics, Warsaw, Poland.
Charles WeigeInternational Institute of Molecular and Cell Biology, Warsaw, Poland; Institute of Biochemistry and Biophysics, Warsaw, Poland.
Michal PastorPolish Academy of Sciences, Institute of Biochemistry and Biophysics, Warsaw, Poland.
Agnieszka KolanoInternational Institute of Molecular and Cell Biology, Warsaw, Poland; Institute of Biochemistry and Biophysics, Warsaw, Poland.
Cecilia WinataInternational Institute of Molecular and Cell Biology, Warsaw, Poland; Institute of Biochemistry and Biophysics, Warsaw, Poland.
Virginijus SiksnysInstitute of Biotechnology, Vilnius University, Vilnius, Lithuania; Institute of Biochemistry and Biophysics, Warsaw, Poland.
Gintautas TamulaitisInstitute of Biotechnology, Vilnius University, Vilnius, Lithuania; Institute of Biochemistry and Biophysics, Warsaw, Poland.
Matthias BochtlerInternational Institute of Molecular and Cell Biology, Warsaw, Poland; Institute of Biochemistry and Biophysics, Warsaw, Poland.
International Institute of Molecular and Cell Biology · PLVilnius University · LTInstitute of Biochemistry and Biophysics, Polish Academy of Sciences · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

RNA interference is a powerful experimental tool for RNA knockdown, but not all organisms are amenable. Here, we provide a proof of principle demonstration that a type III Csm effector complex can be used for programmable mRNA transcript degradation in eukaryotes. In zebrafish,

Indexed as

CRISPR-Cas SystemsRNA StabilityAnimalsAnimals, Genetically ModifiedClustered Regularly Interspaced Short Palindromic RepeatsGene EditingRNA, MessengerStreptococcus thermophilusZebrafishRNA, Messenger

Identifiers

PMID32833532
PMCPMC7469701
OpenAlexW3080535108

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.