Evidence map›Paper›PMID 32833211›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses.

Sarah Keep, Paul Britton, Erica Bickerton

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Sarah KeepThe Pirbright Institute, Surrey, UK.
Paul BrittonThe Pirbright Institute, Surrey, UK.
Erica BickertonThe Pirbright Institute, Surrey, UK. erica.bickerton@pirbright.ac.uk.

Funding

Biotechnology and Biological Sciences Research Council BB/H01425X/1Biotechnology and Biological Sciences Research Council BBS/E/I/00007034
6 · The paper itself

Abstract

We have developed a reverse genetics system for the avian coronavirus infectious bronchitis virus (IBV) in which a full-length cDNA corresponding to the IBV genome is inserted into the vaccinia virus genome under the control of a T7 promoter sequence. Vaccinia virus as a vector for the full-length IBV cDNA has the advantage that modifications can be introduced into the IBV cDNA using homologous recombination, a method frequently used to insert and delete sequences from the vaccinia virus genome. Here, we describe the use of transient dominant selection as a method for introducing modifications into the IBV cDNA that has been successfully used for the substitution of specific nucleotides, deletion of genomic regions, and the exchange of complete genes. Infectious recombinant IBVs are generated in situ following the transfection of vaccinia virus DNA, containing the modified IBV cDNA, into cells infected with a recombinant fowlpox virus expressing T7 DNA-dependent RNA polymerase.

Indexed as

AnimalsBacteriophagesChlorocebus aethiopsDNA-Directed DNA PolymeraseFowlpox virusHomologous RecombinationInfectious bronchitis virusMicroorganisms, Genetically-ModifiedTransfectionVaccinia virusVero Cellsbacteriophage T7 induced DNA polymeraseDNA-Directed DNA PolymeraseAvianCoronavirusFowlpox virusInfectious bronchitis virus (IBV)NidovirusReverse geneticsT7 RNA polymeraseTransient dominant selection (TDS)Vaccinia virus

Identifiers

PMID32833211

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.