Evidence map›Paper›PMID 32833099›Full record

ArticleMikrochimica acta2020

Distance-based quantification of miRNA-21 by the coffee-ring effect using paper devices.

Dagan Zhang, Chao Wu, Chengxin Luan, Peng Gao, Huan Wang, Junjie Chi, Tiantian Kong

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 23 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Dagan ZhangGuangdong Key Laboratory of Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Shenzhen University, Shenzhen, 518060, China.
Chao WuSuzhou Engineering and Technological Research Center of Natural Medicine and Functional Food, School of Biology and Food Engineering, Suzhou University, Suzhou, 234000, China.
Chengxin LuanDepartment of Hematology, the First Affiliated Hospital of Anhui Medical University, Hefei, 230022, Anhui, China.
Peng GaoGuangdong Key Laboratory of Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Shenzhen University, Shenzhen, 518060, China.
Huan WangGuangdong Key Laboratory of Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Shenzhen University, Shenzhen, 518060, China. wang_huan@seu.edu.cn.
Junjie ChiGuangdong Key Laboratory of Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Shenzhen University, Shenzhen, 518060, China. cjj_2337@163.com.
Tiantian KongGuangdong Key Laboratory of Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Shenzhen University, Shenzhen, 518060, China. ttkong@szu.edu.cn.
Shenzhen University · CNFirst Affiliated Hospital of Anhui Medical University · CNSuzhou University of Science and Technology · CN

Funding

Anhui Natural Science Foundation of China 2008085QH365Guangdong Basic and Applied Basic Research Foundation 2019A1515111155Postdoctoral Research Foundation of China 2019M653061Postdoctoral Science Foundation of China 2019M012945Science, Technology and Innovation Commission of Shenzhen Municipality JCYJ20190808120405672Suzhou Engineering and Technological Research Center of Natural Medicine and Functional Food SZ2017ZX06
6 · The paper itself

Abstract

Enabled by the coffee-ring effect, a paper-based signal transduce method is employed for catalytic hairpin assembly (CHA) amplification and hybridization chain reaction (HCR) to achieve miRNA quantification. Once the target miRNAs appeared, it was circularly used by CHA to initiate HCR amplification to produce a large number of G-quadruplex, which is combined with hemin to form a hemin/G-quadruplex DNAzyme. The DNAzyme catalyzes a colorimetric reaction to produce colored nanoparticles, which were converted to the end edge of the paper by evaporation-driven flow, forming a visible colored band. Higher concentration of miRNA led to more colored nanoparticles and thus a longer colored band that can simply be measured by a ruler. The results of determination of miRNA in samples demonstrate that the relative standard deviation of the proposed approach is 5.2%, highly sensitive and repeatable, with a working range 1.0 to 1000 pM and a LOD of 0.2 pM. The paper-based analytical device as a novel platform offers a new signal transduce pathway toward the detection of low-abundance biomarkers for diagnosis.Graphical abstract Schematic representation of the principle for quantification of miRNA on paper based on the coffee-ring effect.

Indexed as

PaperBiomarkers, TumorColorimetryDNA, CatalyticDNA ProbesG-QuadruplexesHeminHumansIodineLimit of DetectionMicroRNAsNucleic Acid Amplification TechniquesBiomarkers, TumorDNA, CatalyticDNA ProbesHeminIodineMicroRNAsMIRN21 microRNA, humanCHA and HCR amplificationCoffee-ring effectLabel-freemiRNA

Identifiers

PMID32833099
OpenAlexW3081426792

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.