ArticleScience advances2020
β-Catenin safeguards the ground state of mousepluripotency by strengthening the robustness of the transcriptional apparatus.
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Article in Science advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
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Who cites it
10 citing papers in PubMed, 16 citations in OpenAlex.
- BRD4 acts as a transcriptional repressor of RhoB to inhibit terminal erythropoiesis.Journal of hematology & oncology · 2025Article
- The nuclear matrix stabilizes primed-specific genes in human pluripotent stem cells.Nature cell biology · 2025Article
- BRD8 Guards the Pluripotent State by Sensing and Maintaining Histone Acetylation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Molecular versatility during pluripotency progression.Nature communications · 2023Review
- Article
- Article
- The mTORC1-eIF4F axis controls paused pluripotency.EMBO reports · 2022Article
- Capture of the newly transcribed RNA interactome using click chemistry.Nature protocols · 2021Article
- Vesicle transporter GOLT1B mediates the cell membrane localization of DVL2 and PD-L2 and promotes colorectal cancer metastasis.Cancer cell international · 2021Article
- Chromatin and Epigenetic Rearrangements in Embryonic Stem Cell Fate Transitions.Frontiers in cell and developmental biology · 2021Review
Corrections and comments
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Authors and funding
56 authors at 16 institutions in 8 countries.
Meng ZhangLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.ORCID 0000-0002-6534-1039
Yiwei LaiLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.ORCID 0000-0003-0733-812X
Vladislav KrupalnikDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
Pengcheng GuoLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Xiangpeng GuoLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.ORCID 0000-0002-3323-1134
Jianguo ZhouLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Yan XuLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Zhijun YuLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.ORCID 0000-0002-1706-118X
Longqi LiuLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Ao JiangHubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan 430072, China.ORCID 0000-0001-6227-4005
Wenjuan LiGuangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou 510005, China.
Mazid Md AbdulLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Gang MaCAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Na LiLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Xiuling FuDepartment of Biology, Southern University of Science and Technology, Shenzhen 518055, China.
Yuan LvLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.ORCID 0000-0003-1260-5800
Mengling JiangLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.ORCID 0000-0002-5034-7382
Muqddas TariqLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.ORCID 0000-0002-6196-3009
Shahzina KanwalLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.ORCID 0000-0003-4694-136X
Hao LiuLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Xueting XuCAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Hui ZhangCAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Yinghua HuangCAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.ORCID 0000-0002-2028-8131
Lulu WangCAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.ORCID 0000-0002-8711-5876
Shuhan ChenLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Isaac A BabarindeDepartment of Biology, Southern University of Science and Technology, Shenzhen 518055, China.
Zhiwei LuoGuangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou 510005, China.ORCID 0000-0002-6628-534X
Dongye WangLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Tiantian ZhouState Key Laboratory of Molecular Biology, Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Carl WardLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.ORCID 0000-0003-0889-9025
Minghui HeForevergen Biosciences Center, Guangzhou 510000, China.
David P IbañezLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Yunpan LiLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Jiajian ZhouDepartment of Chemical Pathology, Li Ka Shing Institute of Health Sciences, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, China.
Jie YuanDepartment of Chemical Pathology, Li Ka Shing Institute of Health Sciences, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, China.
Yayan FengGenome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.ORCID 0000-0002-3932-0779
Karthik ArumugamCentre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona 08003, Spain.
Umberto Di VicinoCentre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona 08003, Spain.ORCID 0000-0001-5209-0982
Xichen BaoLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.ORCID 0000-0003-0389-4233
Guangming WuGuangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou 510005, China.ORCID 0000-0003-1923-7609
Axel SchambachHannover Medical School, Institute of Experimental Hematology, Hannover 30625, Germany.
Huating WangDepartment of Orthopaedics and Traumatology, Li Ka Shing Institute of Health Sciences, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, China.
Hao SunDepartment of Chemical Pathology, Li Ka Shing Institute of Health Sciences, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, China.ORCID 0000-0002-5547-9501
Fei GaoGenome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
Baoming QinCAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Andrew P HutchinsDepartment of Biology, Southern University of Science and Technology, Shenzhen 518055, China.ORCID 0000-0001-7784-2255
Bradley W DobleDepartments of Pediatrics and Child Health and Biochemistry and Medical Genetics, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 0W2, Canada.ORCID 0000-0002-0260-2983
Christine HartmannDepartment of Bone and Skeletal Research, Institute of Musculoskeletal Medicine, Medical Faculty of the University of Münster, Münster D-48149, Germany.ORCID 0000-0002-6854-8633
Maria Pia CosmaCAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.ORCID 0000-0003-4207-5097
Yan QinUniversity of Chinese Academy of Sciences, Beijing 100049, China.
Guo-Liang XuState Key Laboratory of Molecular Biology, Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Runsheng ChenKey Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.ORCID 0000-0001-6049-8347
Giacomo VolpeLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.ORCID 0000-0001-5000-6951
Liang ChenHubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan 430072, China.ORCID 0000-0002-6748-9416
Jacob H HannaDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.ORCID 0000-0003-2042-9974
Miguel A EstebanLaboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.ORCID 0000-0002-1426-6809
Chinese Academy of Sciences · CNChinese University of Hong Kong · HKSouthern University of Science and Technology · CNUniversitat Pompeu Fabra · ESGuangzhou Institutes of Biomedicine and Health · CNWeizmann Institute of Science · ILWuhan University · CNAgricultural Genomics Institute at Shenzhen · CNBoston Children's Hospital · USCenter for Excellence in Molecular Cell Science · CNGuangzhou Regenerative Medicine and Health Guangdong Laboratory · CNJilin University · CNShanghai Medical College of Fudan University · CNUniversity of Copenhagen · DKUniversity of Manitoba · CAUniversity of Münster · DE
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mouse embryonic stem cells cultured with MEK (mitogen-activated protein kinase kinase) and GSK3 (glycogen synthase kinase 3) inhibitors (2i) more closely resemble the inner cell mass of preimplantation blastocysts than those cultured with SL [serum/leukemia inhibitory factor (LIF)]. The transcriptional mechanisms governing this pluripotent ground state are unresolved. Release of promoter-proximal paused RNA polymerase II (Pol2) is a multistep process necessary for pluripotency and cell cycle gene transcription in SL. We show that β-catenin, stabilized by GSK3 inhibition in medium with 2i, supplies transcriptional coregulators at pluripotency loci. This selectively strengthens pluripotency loci and renders them addicted to transcription initiation for productive gene body elongation in detriment to Pol2 pause release. By contrast, cell cycle genes are not bound by β-catenin, and proliferation/self-renewal remains tightly controlled by Pol2 pause release under 2i conditions. Our findings explain how pluripotency is reinforced in the ground state and also provide a general model for transcriptional resilience/adaptation upon network perturbation in other contexts.
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