Evidence map›Paper›PMID 32831656›Full record

ArticleJournal of biomolecular techniques : JBT2020

Method to Overcome Inefficiencies in Site-Directed Mutagenesis of A/T-Rich DNA.

Samuel A DeCero, Christa H Winslow, Jenifer Coburn

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomolecular techniques : JBT, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Frontiers in cellular and infection microbiology · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Samuel A DeCeroDepartment of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, Wisconsin, USA.
Christa H WinslowDepartment of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, Wisconsin, USA.
Jenifer CoburnDepartment of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, Wisconsin, USA.

Funding

Functional Analyses of Borrelia burgdorferi AdhesinsR01AI118799 · NIAID · MEDICAL COLLEGE OF WISCONSIN · PI CHACONAS, GEORGE, COBURN, JENIFER L · 2016 to 2020
$3.3M
Identification of protective Lyme disease antigens using live attenuated vaccinesR01AI121217 · NIAID · MEDICAL COLLEGE OF WISCONSIN · PI COBURN, JENIFER L, NARDELLI, DEAN · 2016 to 2020
$1.9M
NIAID NIH HHS R01 AI118799NIAID NIH HHS R01 AI121217
6 · The paper itself

Abstract

Site-directed mutagenesis (SDM) is an invaluable technique that enables the manipulation of DNA and therefore the primary structure and function of any encoded gene products. Commercial protocols for SDM have been optimized for

Indexed as

AT Rich SequenceDNAEscherichia coliMutagenesis, Site-DirectedOligonucleotidesDNAOligonucleotidesBorrelia burgdorferimutationQ5 DNA PolymeraseQuikChange IIstep-down PCR

Identifiers

PMID32831656
PMCPMC7351327

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.