ArticleJournal of visualized experiments : JoVE2020
Perfusion and Inflation of the Mouse Lung for Tumor Histology.
Article in Journal of visualized experiments : JoVE, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- Maternal low sodium intake and early postnatal diuretics program metabolic and ventilatory dysfunction in mice.Pediatric research · 2026Article
- B cell αv integrin regulates tissue specialization and clonal expansion of lung germinal center and memory B cells after viral infection.Science advances · 2026Article
- Label-free streamlined photoacoustic image guidance facilitates NIR-II photoablation in models of melanoma lung metastases.The Journal of clinical investigation · 2026Article
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- Residual Breast Cancer Cells Co-opt SOX5-Driven Endochondral Ossification to Maintain Dormancy.Cancer discovery · 2026Article
- Histological characterization of major internal organs in infant common marmosets (Callithrix jacchus).PloS one · 2026Article
- Spatial transcriptomic analysis of HIV and tuberculosis coinfection in a humanized mouse model reveals unique transcription patterns, immune responses and early morphological alterations.bioRxiv : the preprint server for biology · 2025Article
- B cell αv integrin regulates tissue specialization and clonal expansion of lung germinal center and memory B cells after viral infection.bioRxiv : the preprint server for biology · 2025Article
- ERC/Mesothelin Is Associated with the Formation of Microvilli on the Mesothelium and Has Limited Functional Relevance Under Physiological Conditions.International journal of molecular sciences · 2025Article
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- Investigating pulmonary neutrophil responses to inflammation in mice via flow cytometry.Journal of leukocyte biology · 2025Article
- Lung contusion complicated by pneumonia worsens lung injury via the inflammatory effect of alveolar small extracellular vesicles on macrophages and epithelial cells.The journal of trauma and acute care surgery · 2025Article
- Immunomodulatory activity of omadacyclinemSphere · 2024Article
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- MicroRNA-210 mediates hypoxia-induced pulmonary hypertension by targeting mitochondrial bioenergetics and mtROS flux.Acta physiologica (Oxford, England) · 2024Article
- Article
- A Novel Humanized Mouse Model for HIV and Tuberculosis Co-infection Studies.bioRxiv : the preprint server for biology · 2024Article
- A novel humanized mouse model for HIV and tuberculosis co-infection studies.Frontiers in immunology · 2024Article
- Bordetella spp. block eosinophil recruitment to suppress the generation of early mucosal protection.Cell reports · 2023Article
- Identification of Virulence Factors Involved in a Murine Model of Severe Achromobacter xylosoxidans Infection.Infection and immunity · 2023Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The ability to evaluate lung histology is critical for the fields of lung cancer research and cancer metastasis. It is equally important to perform necropsies rapidly and efficiently from studies without sacrificing the quality of the tissues procured. The goal of this protocol is to present a method to rapidly perfuse, inflate, and fix mouse lungs for downstream histological analysis. This method does not standardize lung inflation; thus, it does not require any special procedures or equipment and instead simply instills fixative directly through the trachea following perfusion through the heart. This allows for sufficient estimation of tumor size, histology, and scoring. This also allows for the collection of frozen tissue prior to lung tissue fixation. This method is limited in that it does not allow for later morphometric quantification of the lung; however, it is more than sufficient for lung tumor analysis from genetically engineered mouse models (GEMMs), syngeneic models, as well as xenograft tumor and metastasis studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.