ArticleBMC microbiology2020
Influence of biofilm growth age, media, antibiotic concentration and exposure time on Staphylococcus aureus and Pseudomonas aeruginosa biofilm removal in vitro.
Article in BMC microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
53 citing papers in PubMed, 2 syntheses or guidelines pooled it, 97 citations in OpenAlex.
- Antibiofilm efficacy of plant extracts as root canal irrigants in endodontics: a systematic literature review.Frontiers in dental medicine · 2024Pooled it
- A systematic review of the use of bacteriophages for in vitro biofilm control.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2023Pooled it
- Biofilm sensitization to breakJournal of medical microbiology · 2026Article
- A fluorous-phase oxygen optical nanosensor for mitigating redox-active microbial metabolite interference.The Analyst · 2026Article
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- C-BODIPY Dyads Based on Fused-Ring Organoboron Scaffolds for Efficient Photodynamic Therapy TargetingJournal of medicinal chemistry · 2026Article
- High Resistance and Biofilm Tolerance to Antimicrobials of Marine Bacteria From Brazilian Deep-Sea Sediment.Environmental microbiology reports · 2026Article
- Characterising the Antimicrobial Performance of Engineered Layered Double Hydroxide Surfaces for Biofilm Control.Nanomaterials (Basel, Switzerland) · 2026Article
- Design of a Wireless Ultraviolet Germicidal Irradiation System and Validation of Germicidal Potential Against Biofilm-Forming Bacteria and Fungi.Antibiotics (Basel, Switzerland) · 2026Article
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- The modified-KLICC score: a novel tool to predict outcomes following debridement, antibiotics, and implant retention after early acute periprosthetic hip infection.Bone & joint open · 2025Article
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- Development and Physico-Chemical and Antibacterial Characterization of Chromium-Doped Hydroxyapatite in a Chitosan Matrix Coating.Polymers · 2025Article
- Biofilm Eradication Using Water-Soluble Silver(I) Coumarin Complexes.Chembiochem : a European journal of chemical biology · 2025Article
- Trypan Blue Image-Guided Removal of Surface-Based Bacterial Biofilms from Chicken Tissue Using Cold Atmospheric Pressure Plasma.Plasma (Basel, Switzerland) · 2025Article
- Efficacy of nitric oxide donors and EDTA againstBiofilm · 2025Article
- Proteomic signatures ofBiofilm · 2025Article
- Enhancing biofilm disruption and bactericidal efficiency using vancomycin-loaded microbubbles in sonodynamic therapy.JAC-antimicrobial resistance · 2025Article
- Struggling with a cefazolin impregnation protocol of bone chips.Cell and tissue banking · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundBiofilm is known to be tolerant towards antibiotics and difficult to eradicate. Numerous studies have reported minimum biofilm eradication concentration (MBEC) values of antibiotics for many known biofilm pathogens. However, the experimental parameters applied in these studies differ considerably, and often the rationale behind the experimental design are not well described. This makes it difficult to compare the findings. To demonstrate the importance of experimental parameters, we investigated the influence of biofilm growth age, antibiotic concentration and treatment duration, and growth media on biofilm eradication. Additionally, OSTEOmycin™, a clinically used antibiotic containing allograft bone product, was tested for antibiofilm efficacy.
resultsThe commonly used Calgary biofilm device was used to grow 24 h and 72 h biofilms of Staphylococcus aureus and Pseudomonas aeruginosa, which were treated with time-dependent vancomycin (up to 3000 mg L
conclusionsThis study demonstrated biofilm removal efficacy was influenced by media, biofilm age and antibiotic concentration and treatment duration. It is therefore necessary to taking these parameters into consideration when designing experiments. The results of OSTEOmycin™ products indicated that simple in vitro biofilm test could be used for initial screening of antibiofilm products. For clinical application, a more clinically relevant biofilm model for the specific biofilm infection in question should be developed to guide the amount of antibiotics used for local antibiofilm treatment.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.