ArticleBMC genomics2020
Functional characterization and regulatory mechanism of wheat CPK34 kinase in response to drought stress.
Article in BMC genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 20 citations in OpenAlex.
- Integrative Physiological, Transcriptomic, and Functional Analysis Reveals a Positive Contribution ofGenes · 2026Article
- Integrative transcriptional regulatory networks governing cereal root responses to heavy metals and drought.Plant cell reports · 2026Review
- Advances in gene cloning and functional genomics approaches for wheat (Triticum aestivum L.) improvement.The plant genome · 2026Review
- Drought-tolerant wheat for enhancing global food security.Functional & integrative genomics · 2024Review
- Accurate Long-Read RNA Sequencing Analysis Reveals the Key Pathways and Candidate Genes under Drought Stress in the Seed Germination Stage in Faba Bean.International journal of molecular sciences · 2024Article
- Modulation of the wheat transcriptome by TaZFP13D under well-watered and drought conditions.Plant molecular biology · 2024Article
- Caffeine Produced in Rice Plants Provides Tolerance to Water-Deficit Stress.Antioxidants (Basel, Switzerland) · 2023Article
- Silencing of the calcium-dependent protein kinase TaCDPK27 improves wheat resistance to powdery mildew.BMC plant biology · 2023Article
- Genome-Wide Identification and Analysis of FKBP Gene Family in Wheat (International journal of molecular sciences · 2022Article
- The Calcium-Dependent Protein KinaseInternational journal of molecular sciences · 2022Article
- The BBX geneHorticulture research · 2022Article
- iTRAQ based protein profile analysis revealed key proteins involved in regulation of drought-tolerance during seed germination in Adzuki bean.Scientific reports · 2021Article
- An EfficientFrontiers in plant science · 2021Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDrought is one of the most adverse environmental factors limiting crop productions and it is important to identify key genetic determinants for food safety. Calcium-dependent protein kinases (CPKs) are known to be involved in plant growth, development, and environmental stresses. However, biological functions and regulatory mechanisms of many plant CPKs have not been explored. In our previous study, abundance of the wheat CPK34 (TaCPK34) protein was remarkably upregulated in wheat plants suffering from drought stress, inferring that it could be involved in this stress. Therefore, here we further detected its function and mechanism in response to drought stress.
resultsTranscripts of the TaCPK34 gene were significantly induced after PEG-stimulated water deficiency (20% PEG6000) or 100 μM abscisic acid (ABA) treatments. The TaCPK34 gene was transiently silenced in wheat genome by using barley stripe mosaic virus-induced silencing (BSMV-VIGS) method. After 14 days of drought stress, the transiently TaCPK34-silenced wheat seedlings showed more sensitivity compared with control, and the plant biomasses and relative water contents significantly decreased, whereas soluble sugar and MDA contents increased. The iTRAQ-based quantitative proteomics was employed to measure the protein expression profiles in leaves of the transiently TaCPK34-silenced wheat plants after drought stress. There were 6103 proteins identified, of these, 51 proteins exhibited significantly altered abundance, they were involved in diverse function. And sequence analysis on the promoters of genes, which encoded the above identified proteins, indicated that some promoters harbored some ABA-responsive elements. We determined the interactions between TaCPK34 and three identified proteins by using bimolecular fluorescent complementation (BiFC) method and our data indicated that TaCPK34directly interacted with the glutathione S-transferase 1 and prx113, respectively.
conclusionsOur study suggested that the TaCPK34 gene played positive roles in wheat response to drought stress through directly or indirectly regulating the expression of ABA-dependent manner genes, which were encoding identified proteins from iTRAQ-based quantitative proteomics. And it could be used as one potential gene to develop crop cultivars with improved drought tolerance.
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